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12-347 Sigma-AldrichHistone H4 Peptide

Histone H4 Peptide primarily used in Histone Acetyltransferase Assays (HAT) and Western Blotting.

Histone H4 Peptide MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Key Applications: Histone Acetyltransferase Assay (HAT), Western Blotting UniProt Number: P62805 Entrez Gene Number: NM_003538.3

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12-347

100 µg

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Key Spec Table

Key Applications	Entrez Gene Number	Uni Prot Number
HAT, WB	NM_003538.3	P62805

Description
Catalogue Number	12-347
Brand Family	Upstate
Trade Name	Upstate
Description	Histone H4 Peptide

References

Product Information
Presentation	Lyophilized powder
Quality Level	MQ100

Applications
Application	Histone H4 Peptide primarily used in Histone Acetyltransferase Assays (HAT) and Western Blotting.
Key Applications	Histone Acetyltransferase Assay (HAT) Western Blotting

Biological Information
Entrez Gene Number	NM_003538.3
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Gene Symbol	HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH HIST1H4H H4F2 H4FM H4/A H4FK H4FG HIST2H4 H4FB H4/K HIST1H4I H4/N HIST1H4B H4FE H4/M H4/a HIST1H4E HIST4H4 HIST2H4A H4FC H4FI H4/H HIST1H4C H4/G HIST1H4K H4FJ H4FD H4/I H4/B H4/D H4/C HIST1H4J HIST1H4D H4/E
UniProt Number	P62805
UniProt Summary	FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Immunoblot Analysis. This peptide can be used as a standard for antibodies specific for the N-terminus of histone H4. It can also be chemically acetylated with [³H]CH₃COONa using the HDAC Assay Kit (17-281). The radiolabeled peptide was used to assay HDAC activity in a HeLa nuclear extract (12-309).
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	2 years at -20°C

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
12-347	04053252334504

Documentation

Histone H4 Peptide SDS

Title
Safety Data Sheet (SDS)

Histone H4 Peptide Certificates of Analysis

Title	Lot Number
Histone H4 Peptide	2487892
Histone H4 Peptide (substrate for HAT activity) - 2459574	2459574
Histone H4 Peptide (substrate for HAT activity)	2964463
Histone H4 Peptide - 16515	16515
Histone H4 Peptide - 18894	18894
Histone H4 Peptide - 20638	20638
Histone H4 Peptide - 21828	21828
Histone H4 Peptide - 3319768	3319768
Histone H4 Peptide - 3944858	3944858
Histone H4 Peptide - 4179410	4179410

References

Reference overview	Pub Med ID
The TAF(II)250 subunit of TFIID has histone acetyltransferase activity. Mizzen, C A, et al. Cell, 87: 1261-70 (1996) 1996 Show Abstract The transcription initiation factor TFIID is a multimeric protein complex composed of TATA box-binding protein (TBP) and many TBP-associated factors (TAF(II)s). TAF(II)s are important cofactors that mediate activated transcription by providing interaction sites for distinct activators. Here, we present evidence that human TAF(II)250 and its homologs in Drosophila and yeast have histone acetyltransferase (HAT) activity in vitro. HAT activity maps to the central, most conserved portion of dTAF(II)230 and yTAF(II)130. The HAT activity of dTAF(II)230 resembles that of yeast and human GCN5 in that it is specific for histones H3 and H4 in vitro. Our findings suggest that targeted histone acetylation at specific promoters by TAF(II)250 may be involved in mechanisms by which TFIID gains access to transcriptionally repressed chromatin.	8980232

Reference overview

Pub Med ID

Related Products & Applications

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Catalogue Number

Description

12-405

Histone H4 Peptide, biotin conjugate

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12-347 Sigma-AldrichHistone H4 Peptide

Histone H4 Peptide primarily used in Histone Acetyltransferase Assays (HAT) and Western Blotting.

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