Millipore Sigma Vibrant Logo

12-357 Histone H3 Peptide

This gene contains introns & its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

Histone H3 Peptide MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays UniProt Number: Q16695 Entrez Gene Number: NM_002107.3
View Products on Sigmaaldrich.com
12-357
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      Special Shipping Offer on Antibodies
      100% Performance Guaranteed

      Key Spec Table

      Key ApplicationsEntrez Gene NumberUni Prot Number
      Enzyme AssaysNM_002107.3 Q16695
      Description
      Catalogue Number12-357
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHistone H3 Peptide
      References
      Product Information
      PresentationLyophilized powder
      Quality LevelMQ100
      Applications
      ApplicationThis gene contains introns & its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Key Applications
      • Enzyme Assays
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in a Dot Blot Assay by spotting on nitrocellulose and detecting with polyclonal anti-Histone H3 (06-755).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      12-357 04053252362101

      Documentation

      Histone H3 Peptide SDS

      Title

      Safety Data Sheet (SDS) 

      Histone H3 Peptide Certificates of Analysis

      TitleLot Number
      Histone H3 Peptide (residues 1-20) - 2352348 2352348
      Histone H3 Peptide (residues 1-20) - 2376675 2376675
      Histone H3 Peptide (residues 1-20) 3079177
      Histone H3 Peptide - 2450011 2450011
      Histone H3 Peptide - 18691 18691
      Histone H3 Peptide - 2021776 2021776
      Histone H3 Peptide - 22542 22542
      Histone H3 Peptide - 24998 24998
      Histone H3 Peptide - 2943600 2943600
      Histone H3 Peptide - 32623 32623
      Show more

      References

      Reference overviewPub Med ID
      Effects of a novel arginine methyltransferase inhibitor on T-helper cell cytokine production.
      Bonham, K; Hemmers, S; Lim, YH; Hill, DM; Finn, MG; Mowen, KA
      The FEBS journal  277  2096-108  2010

      Show Abstract Full Text Article      		20345902 20345902

      Related Products & Applications

      Alternative Format

      Catalogue Number Description  
      12-403 Histone H3 Peptide, biotin conjugate Show Pricing & Availability

      Product Families

      Recombinant Histone Proteins, Enzymes, Peptides & Inhibitors

      Purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates and inhibitors.
      Learn More >>

      Histone H3 Antibodies, Proteins and Kits

      Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
      Learn More >>

      Categories

      Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Control Histones