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109218 Gram's crystal violet solution

for the Gram staining method

Gram's crystal violet solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109218
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Overview

Replacement Information

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      		 Plastic bottle 500 ml
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      1092182500
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          		 Plastic bottle 2.5 l
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          Description
          Catalogue Number109218
          DescriptionGram's crystal violet solution
          OverviewGram's crystal violet solution for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with Gram's crystal violet solution (Cat. No.109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution stabilized with PVP, Cat No.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. No. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Gram's safranine solution (Cat.no. 109217) and will then appear pink to red.
          A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor the Gram staining method
          Biological Information
          Physicochemical Information
          Density0.99 g/cm3 (20 °C)
          Flash point47 °C
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Statement(s)H226: Flammable liquid and vapour.
          H412: Harmful to aquatic life with long lasting effects.
          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
          P233: Keep container tightly closed.
          P240: Ground and bond container and receiving equipment.
          P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
          P242: Use non-sparking tools.
          P273: Avoid release to the environment.
          Signal WordWarning
          Storage class3 Flammable liquids
          WGKWGK 2 obviously hazardous to water
          Disposal28
          Aqueous solutions: Container D.
          Safety Information
          Categories of dangerflammable, dangerous for the environment
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Declaration (railroad and road) ADR, RIDKein Gefahrgut
          Declaration (transport by air) IATA-DGRNo Dangerous Good
          Declaration (transport by sea) IMDG-CodeNo Dangerous Good
          Supplemental Information
          Specifications
          Suitability for microscopypasses test
          gram-positve microorganismsblue-violet
          gram-negative microorganismspink to red
          Global Trade Item Number
          Catalogue Number GTIN
          1092180500 04022536112077
          1092182500 04022536557403

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          Accessories (Primary)

          Catalogue Number Description  
          100567 Lugol's solution Show Pricing & Availability
          109217 Gram's safranine solution Show Pricing & Availability
          109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
          110218 Gram's decolorizing solution Show Pricing & Availability

          Accessories (Secondary)

          Catalogue Number Description  
          100567 Lugol's solution Show Pricing & Availability
          109217 Gram's safranine solution Show Pricing & Availability
          109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
          111885 Gram-Color Show Pricing & Availability

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          Categories

          IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining