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AP500A Goat Anti-Mouse IgM µ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed

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AP500A
1 mL  
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      Overview

      Description
      Catalogue NumberAP500A
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Mouse IgM µ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed
      Background InformationIgM constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.
      Product Information
      FormatAlkaline Phosphatase
      PresentationPurified by affinity chromatography on mouse IgM covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.
      Quality LevelMQ100
      Applications
      ApplicationGoat anti-Mouse IgM µ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed detects level of Mouse IgM µ chain & has been published & validated for use in ELISA.
      Key Applications
      • ELISA
      Application NotesELISA: 1:2,000-1:4,000

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPrepared from purified mouse IgM µ chain. Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.
      EpitopeIgM µ chain
      HostGoat
      SpecificityThe antibody reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
      Species Reactivity
      • Mouse
      Species Reactivity NoteReacts with heavy chain of mouse IgM. Absorbed for mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Chromatography
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size1 mL
      Global Trade Item Number
      Catalogue Number GTIN
      AP500A 08436037124082