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346774 Glucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides

Glucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides, CAS 9001-40-5, catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconate using NAD+ or NADP+.

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346774
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      Description
      OverviewNative glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides. Catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconate. The enzyme can use either NAD+ or NADP+ as an electron acceptor. Reaction velocity with NAD+ is ~1.8 times greater than that with NADP+. Note: 10,000 NADP units are equivalent to ~18,000 NAD units.
      Catalogue Number346774
      Brand Family Calbiochem®
      References
      ReferencesLevy, H.R. 1989. Biochem. Soc. Trans. 17, 313.
      Product Information
      CAS number9001-40-5
      Unit of DefinitionOne unit is defined as the amount of enzyme that will reduce 1.0 µmol NADP per min at 30°C, pH 7.8.
      EC number1.1.1.49
      FormLyophilized
      Quality LevelMQ100
      Applications
      ApplicationGlucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides, CAS 9001-40-5, catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconate using NAD+ or NADP+.
      Biological Information
      Specific Activity≥200 units/mg protein
      Physicochemical Information
      ContaminantsPhosphohexose isomerase: ≤0.02%;
      Creatine phosphokinase: ≤0.002%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      346774-500U 04055977193169

      Documentation

      Glucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides SDS

      Title

      Safety Data Sheet (SDS) 

      Glucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides Certificates of Analysis

      TitleLot Number
      346774

      References

      Reference overview
      Levy, H.R. 1989. Biochem. Soc. Trans. 17, 313.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-September-2007 RFH
      DescriptionNative glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides. Catalyzes the conversion of glucose-6-phosphate to 6-phospho-gluconate. The enzyme can use either NAD or NADP+ as an electron acceptor. Reaction velocity with NAD is approximately 1.8 times greater thant that with NADP+.
      FormLyophilized
      Recommended reaction conditions50 mM Tris-HCl pH 7.8 containing 3 mM MgCl2.
      CAS number9001-40-5
      EC number1.1.1.49
      ContaminantsPhosphohexose isomerase: ≤0.02%;
      Creatine phosphokinase: ≤0.002%
      Specific activity≥200 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will reduce 1.0 µmol NADP per min at 30°C, pH 7.8.
      SolubilityReconstitute to 1 mg/ml in 5 mM glycine buffer, pH 8.0. Agitation of solution may cause precipitation.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
      Toxicity Standard Handling
      ReferencesLevy, H.R. 1989. Biochem. Soc. Trans. 17, 313.