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324762 Endopeptidase, Neutral, Porcine Kidney

Endopeptidase, Neutral, Porcine Kidney MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin

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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Native, neutral endopeptidase from porcine kidney. A cell surface, zinc containing metallopeptidase that is abundant in the brush border membrane of the kidney proximal tubules. Also present in brain, endothelial cells, liver, and lung. Peptidase activity is specifically directed toward the cleavage on the amino side of hydrophobic residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.
      Catalogue Number324762
      Brand Family Calbiochem®
      SynonymsCD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin
      References
      ReferencesBarnes, K., et al. 1995. J. Neurochem. 64, 1826.
      Gafford, J.T., et al. 1983. Biochemistry 22, 3265.
      Product Information
      Unit of DefinitionOne unit of enzyme will liberate 1.0 µmol <i>p</i>NA per min from Glutaryl-(Ala)₂-Phe-<i>p</i>NA at 25°C, pH 7.4.
      EC number3.4.24.11
      FormLyophilized
      FormulationLyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON® X-100 Detergent, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Specific Activity≥850 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      324762 0

      Documentation

      Endopeptidase, Neutral, Porcine Kidney SDS

      Title

      Safety Data Sheet (SDS) 

      Endopeptidase, Neutral, Porcine Kidney Certificates of Analysis

      TitleLot Number
      324762

      References

      Reference overview
      Barnes, K., et al. 1995. J. Neurochem. 64, 1826.
      Gafford, J.T., et al. 1983. Biochemistry 22, 3265.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      SynonymsCD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin
      DescriptionNative, neutral endopeptidase from porcine kidney. A cell surface zinc-containing proteinase that is abundant in the brush border membranes of the kidney proximal tubules. Also present in the brain, endothelial cells, liver, and the lung tissue. Peptidase activity is specifically directed towards the cleavage on the amino side of hydrophobic amino acid residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.
      FormLyophilized
      FormulationLyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON® X-100 Detergent, pH 7.4.
      Recommended reaction conditionsSee Gafford, J. T., et al. 1983 (below)
      EC number3.4.24.11
      Purity≥95% by HPLC
      Specific activity≥850 units/mg protein
      Unit definitionOne unit of enzyme will liberate 1.0 µmol pNA per min from Glutaryl-(Ala)₂-Phe-pNA at 25°C, pH 7.4.
      SolubilityPhysiological buffers, pH 7.4 (e.g., Tris-buffered saline) (1 mg/ml)
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.
      Toxicity Standard Handling
      ReferencesBarnes, K., et al. 1995. J. Neurochem. 64, 1826.
      Gafford, J.T., et al. 1983. Biochemistry 22, 3265.