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L2113-BC EDTA (Versen), 1 %

L2113-BC
  
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      Overview

      Replacement Information
      Description
      OverviewTrypsin/EDTA solutions (0.05 or 0.02% in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum.
      Specifications:

      Functional Test: Pass
      Activity Test: Pass
      pH: 7.2-7.5
      Osmolality: 0.27-0.30 Osm/kg H20
      Color: Colorless
      Appearance: Clear
      Recommendation Instructions:

      Recommendations Trypsin (1:250)/EDTA solutions:
      Trypsin (1:250)/EDTA solutions (0.05/0.02 % in PBS without Ca2+, Mg2+) to passage monolayer cultures:
      1. Wash cell culture (monolayer) using physiological saline (w/o Ca2+, Mg2+).
      2. Apply a small quantity of Trypsin (1:250)/EDTA solution with a pipette to cell culture flask; a thin film is sufficient.
      3. Incubate culture flask at +37°C for approx. 5 min.
      4. Centrifuge cell suspension or transfer directly to a serum containing culture medium.

      With higher Trypsin concentrations, it is recommended to trypsinate at +2-+8°C.
      Catalogue NumberL2113-BC
      Brand Family Calbiochem®
      References
      Product Information
      Quality LevelMQ300
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      L2113-BC 0

      Documentation