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292505 Diethylamine NONOate/AM - CAS 213768-16-2 - Calbiochem

An esterase-sensitive and cell permeable, O²-acetoxymethylated diazeniumdiolate that acts as an intracellular nitric oxide (NO) donor (1.83 mol of NO/mol).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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292505
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
213768-16-2C₇H₁₅N₃O₄

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292505-1MG
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      Description
      OverviewAn esterase-sensitive and cell permeable, O2-acetoxymethylated diazeniumdiolate that acts as an intracellular nitric oxide (NO) donor (1.83 mol of NO/mol). Shown to induce apoptosis and to act as an antiproliferative agent in NO-sensitive human leukemia HL-60 (IC50 = 8.3 µM) and U937 (IC50 = 53 µM) cell lines. Also suppresses oxidized low-density lipoprotein-induced caspase-3 activation in bovine aortic endothelial cells. Exhibits enhanced stability in physiological buffers. May also be useful for temporal and spatial controlled NO availability.
      Catalogue Number292505
      Brand Family Calbiochem®
      References
      ReferencesKotamraju, S., et al. 2001. J. Biol. Chem. 276, 17316.
      Saavedra, J.E., et al. 2000. J. Med. Chem. 43, 261.
      Product Information
      CAS number213768-16-2
      ATP CompetitiveN
      FormYellow liquid
      Hill FormulaC₇H₁₅N₃O₄
      Chemical formulaC₇H₁₅N₃O₄
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetIntracellular nitric oxide (NO) donor
      Primary Target IC<sub>50</sub>8.3 µM, 53 µM, acting as an antiproliferative agent in NO-sensitive human leukemia HL-60 and U937 cell lines, respectively
      Purity≥95% by NMR
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 2 months at -20°C. Add to aqueous medium just prior to use.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      292505-1MG 04055977197945

      Documentation

      Diethylamine NONOate/AM - CAS 213768-16-2 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Diethylamine NONOate/AM - CAS 213768-16-2 - Calbiochem Certificates of Analysis

      TitleLot Number
      292505

      References

      Reference overview
      Kotamraju, S., et al. 2001. J. Biol. Chem. 276, 17316.
      Saavedra, J.E., et al. 2000. J. Med. Chem. 43, 261.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-April-2011 RFH
      DescriptionA cell-permeable O2-acetoxymethylated diazeniumdiolate that donates nitric oxide (NO) following the action of intracellular esterases (1.83 mol of NO/mol). Shown to induce apoptosis and act as an antiproliferative agent in NO-sensitive human leukemia HL-60 (IC50 = 8.3 µM) and U937 (IC50 = 53 µM) cell lines. Also suppresses oxidized low-density lipoprotein-induced caspase-3 activation (~5 µM) in bovine aortic endothelial cells. Useful for temporal and spatial controlled NO availability.
      FormYellow liquid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number213768-16-2
      Chemical formulaC₇H₁₅N₃O₄
      Structure formulaStructure formula
      Purity≥95% by NMR
      SolubilityDMSO (5 mg/ml)
      Storage Protect from moisture
      Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 2 months at -20°C. Add to aqueous medium just prior to use.
      Toxicity Standard Handling
      ReferencesKotamraju, S., et al. 2001. J. Biol. Chem. 276, 17316.
      Saavedra, J.E., et al. 2000. J. Med. Chem. 43, 261.