Millipore Sigma Vibrant Logo

2114 Detergent-OUT™ Micro Kit

The Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions.

Detergent-OUT™ Micro Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Protein Clean-up
2114
1 kit  10 columns
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Key Spec Table

      Key Applications
      PCU
      Description
      Catalogue Number2114
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDetergent-OUT™ Micro Kit
      OverviewThe Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. Micro columns are suitable for removing detergent from a maximum of 0.2 mL sample volume.
      Materials Required but Not DeliveredCentrifuge

      Centrifuge tubes
      Product Information
      Components
      • Detergent-OUT Columns-DTG-100X 10 Micro
      • Collection Tubes (2 mL) 10
      Applications
      ApplicationThe Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions.
      Key Applications
      • Protein Clean-up
      Application NotesPROTOCOL:

      1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.

      2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with Tris buffer. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.

      3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.

      4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 1-2 minutes. Note: If your protein solution is in Tris buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT column or by dialysis.

      5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
      Packaging Information
      Material Size1 kit
      Material Package10 columns
      Global Trade Item Number
      Catalogue Number GTIN
      2114 04053252278426