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QIA87 Cytochrome c Release Apoptosis Assay Kit

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QIA87
  
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      Overview

      Description
      Overview

      This product has been discontinued.



      Assay kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. Translocation of cytochrome c from the mitochondrial fraction to the cytosol is monitored by immunoblotting with the cytochrome c antibody provided with the kit.

      Catalogue NumberQIA87
      Brand Family Calbiochem®
      Materials Required but Not Delivered DMSO
      PBS
      Product Information
      Form100 Tests
      FormatImmunoblot
      Kit containsMitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, Cytochrome c Antibody, and a user protocol.
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Biological Information
      Assay time4 h
      Sample TypeCell Extracts
      Safety Information
      R PhraseR: 32-23/24/25-34-36/37/38-41

      Contact with acids liberates very toxic gas.
      Toxic by inhalation, in contact with skin and if swallowed.
      Causes burns.
      Irritating to eyes, respiratory system and skin.
      Risk of serious damage to eyes.
      S PhraseS: 23-26-36/37/39-45

      Do not breathe fumes.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Intended useThe Cytochrome c Release Apoptosis Assay Kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol that can be used to detect cytochrome c by Western blotting.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Storage ConditionsUpon arrival store the entire contents of the kit at -20°C. Once opened, store the Mitochondria Extraction Buffer and Cytosol Extraction Buffer at 4°C and the remaining components at -20°C.
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsMitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, Cytochrome c Antibody, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      QIA87 0