Millipore Sigma Vibrant Logo

115940 Crystal violet (C.I. 42555)

View Products on Sigmaaldrich.com
115940
View Pricing & Availability

Overview

Replacement Information
Description
Catalogue Number115940
SynonymsHexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B
DescriptionCrystal violet (C.I. 42555)
OverviewCrystal violet (C.I. 42555) for microscopy Certistain®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The CERTISTAIN® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
CAS number548-62-9
EC index number612-204-00-2
EC number208-953-6
Hill FormulaC₂₅H₃₀ClN₃
Molar Mass407.99 g/mol
HS Code3212 90 00
Structure formula ImageStructure formula Image
Quality LevelMQ400
Applications
Applicationfor microscopy Certistain®
Biological Information
Physicochemical Information
Density1.19 g/cm3 (20 °C)
Melting Point215 °C
pH value2.5 - 3.5 (10 g/l, H₂O, 20 °C)
Bulk density220 - 400 kg/m3
Solubility10 g/l
Dimensions
Materials Information
Toxicological Information
LD 50 oralLD50 Rat 420 mg/kg
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H302: Harmful if swallowed.
H318: Causes serious eye damage.
H351: Suspected of causing cancer.
H410: Very toxic to aquatic life with long lasting effects.
Precautionary Statement(s)P202: Do not handle until all safety precautions have been read and understood.
P273: Avoid release to the environment.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P308 + P313: IF exposed or concerned: Get medical advice/ attention.
Signal WordDanger
RTECSBO9000000
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal9
Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
Safety Information
Categories of dangerharmful, carcinogenic, irritant, dangerous for the environment
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 3077 , 9, III
Declaration (transport by air) IATA-DGRUN 3077 , 9, III
Declaration (transport by sea) IMDG-CodeUN 3077 , 9, III, Marine Pollutant: P
Supplemental Information
Specifications
Dye content (spectrophotometrically)≥ 88 %
Identity (UV/VIS-Spectrum)passes test
Absorption maximum λ max (water)589 - 594 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water)2050 - 2450
TLC-Testpasses test
Michler`s ketone< 0.10 %
Loss on drying (110 °C)≤ 10 %
Suitability for microscopypasses test