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234401 Complement C1q-Depleted Serum, Human

Complement C1q-Depleted Serum, Human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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234401
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234401-1ML
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      Description
      OverviewHuman serum that has been depleted of C1q complement component.
      Catalogue Number234401
      Brand Family Calbiochem®
      References
      Product Information
      Unit of Definition<b>Classical Pathway Activity:</b> One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. <b>CH50 Functional Activity upon Reconstitution:</b> One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. <b>Alternative Pathway Activity:</b> One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
      FormClear, amber solution
      FormulationIn PBS, ≤0.1% sodium azide, pH 7.4.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application CommentsNo C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:

      CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.
      • Recommended volume of C1q-Dpl serum per assay
      • C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl
      • Input of purified C1q to yield 1 C1qH50:
      • C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl
      • Input of C1q in NHS Complement standard to yield 1 C1qH50
      • Ratio
      • Background A412 EA blank reading at the recommended input of C1q-Dpl

      CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.
      • CH50/ml NHS Standard
      • CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:
      • Ratio

      ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
      • APH50/ml NHS Standard
      • Input of NHS Standard to yield 1 APH50
      • APH50/ml C1q-Dpl
      • Input of C1q-Dpl to yield 1 APH50
      • AP Ratio
      Biological Information
      SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsC1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      234401-1ML 04055977200195

      Documentation

      Complement C1q-Depleted Serum, Human SDS

      Title

      Safety Data Sheet (SDS) 

      Complement C1q-Depleted Serum, Human Certificates of Analysis

      TitleLot Number
      234401

      Citations

      Title
    • Paramita Baruah, et al. (2006) The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells. Journal of Leukocyte Biology in press,.
    • Jose Yuste, et al. (2006) Roles of the Alternative Complement Pathway and C1q during Innate Immunity to Streptococcus pyogenes. Journal of Immunology 176, 6112-6120.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-June-2008 RFH
      DescriptionHuman serum that has been depleted of C1q complement component. C1q-depleted human serum, sterile filtered through a 0.22 µm filter. This product has the following specifications:
      FormClear, amber solution
      FormulationIn PBS, ≤0.1% sodium azide, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditionsClassical Pathway Activity Recommended volume C1q-Dpl serum per assay: ≤ 40 µl C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl: ≥ 12,500 C1qH50/mg C1q input/C1qH50: ≤80 ng C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl: ≥3,500 units/ml Input of C1q in NHS Complement standard to yield 1 C1qH50: ≤ 20 ng C1q in NHS Standard Ratio: ≥ 0.20 (C1qH50/mg C1q)/(C1qH50/mg C1q in NHS Std) Background A412 EA blank reading at the recommended input of C1q-Dpl: ≤ 0.250 CH50 Functional Activity upon Reconstitution CH50/ml NHS Standard: ≥75 CH50 units/ml CH50/ml C1q-Dpl reconstituted with 100 µg C1q/ml: ≥40 CH50 units/ml Ratio: ≥0.50 (CH50/ml C1q-Dpl + C1q)/(CH50/ml NHS Std) Alternative Pathway Activity APH50 units/ml NHS Complement Standard: ≥50 APH50 units/ml Input of NHS Standard to yield 1 APH50: ≤20 µl APH50 units/ml C1q-Dpl: ≥50 APH50 units/ml Input of C1q-Dpl to yield 1 APH50: ≤20 µl AP Ratio: ≥0.50 (APH50/ml C1q-Dpl)/(APH50/ml NHS Std)
      SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
      ContaminantsC1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.
      Unit definitionClassical Pathway Activity: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
      PreservativeNone
      CommentsNo C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:

      CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.
      • Recommended volume of C1q-Dpl serum per assay
      • C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl
      • Input of purified C1q to yield 1 C1qH50:
      • C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl
      • Input of C1q in NHS Complement standard to yield 1 C1qH50
      • Ratio
      • Background A412 EA blank reading at the recommended input of C1q-Dpl

      CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.
      • CH50/ml NHS Standard
      • CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:
      • Ratio

      ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
      • APH50/ml NHS Standard
      • Input of NHS Standard to yield 1 APH50
      • APH50/ml C1q-Dpl
      • Input of C1q-Dpl to yield 1 APH50
      • AP Ratio
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      Citation
    • Paramita Baruah, et al. (2006) The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells. Journal of Leukocyte Biology in press,.
    • Jose Yuste, et al. (2006) Roles of the Alternative Complement Pathway and C1q during Innate Immunity to Streptococcus pyogenes. Journal of Immunology 176, 6112-6120.
      		•