Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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234184
Sigma-AldrichCollagen, Type II, Bovine Joint Cartilage
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Description
Overview
Highly purified preparation isolated from the triple helical domain of guanidine-HCl-extracted and pepsin-digested bovine joint cartilage collagen Type II. Subunit composition: α1(II)3. Does not react with anti-collagen Type I and anti-collagen Type III by Western blotting.
Catalogue Number
234184
Brand Family
Calbiochem®
References
References
Sieper, J., et al. 1996. Arthritis Rheum. 39, 41. Boissier, M.-C., et al. 1990. Arthritis Rheum. 33, 1.
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C. Avoid freeze/thaw cycles of solutions.
Collagen, Type II, Bovine Joint Cartilage Certificates of Analysis
Title
Lot Number
234184
References
Reference overview
Sieper, J., et al. 1996. Arthritis Rheum. 39, 41. Boissier, M.-C., et al. 1990. Arthritis Rheum. 33, 1.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
29-April-2008 RFH
Description
Highly purified preparation isolated from the triple helical domain of guanidine-HCl-extracted and pepsin-digested bovine joint cartilage collagen Type II. Subunit composition: α1(II)3.
Form
Salt-free lyophilized solid
CAS number
9007-34-5
Purity
≥95% by SDS-PAGE
Solubility
500 mM Acetic acid (1 mg/ml). Solutions gradually solidify at room temperature.
Storage
-20°C Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
Merck USA index
14, 2480
References
Sieper, J., et al. 1996. Arthritis Rheum. 39, 41. Boissier, M.-C., et al. 1990. Arthritis Rheum. 33, 1.
