CellASIC® ONIX Microfluidic Platform
This CellASIC System delivers breakthrough control for live cell analysis experiments by combining the highest precision controls, maximum functionality, and simplified user operation.
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This CellASIC System delivers breakthrough control for live cell analysis experiments by combining the highest precision controls, maximum functionality, and simplified user operation. Less
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Overview
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Documentation
References
| Reference overview | Pub Med ID |
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| Heritable change caused by transient transcription Gordon AJ, Satory D, Halliday JA, Herman C PLoS Genetics 9 (6): e1003595 2013 Show Abstract Abstract Transmission of cellular identity relies on the faithful transfer of information from the mother to the daughter cell. This process includes accurate replication of the DNA, but also the correct propagation of regulatory programs responsible for cellular identity. Errors in DNA replication (mutations) and protein conformation (prions) can trigger stable phenotypic changes and cause human disease, yet the ability of transient transcriptional errors to produce heritable phenotypic change (‘epimutations’) remains an open question. Here, we demonstrate that transcriptional errors made specifically in the mRNA encoding a transcription factor can promote heritable phenotypic change by reprogramming a transcriptional network, without altering DNA. We have harnessed the classical bistable switch in the lac operon, a memory-module, to capture the consequences of transient transcription errors in living Escherichia coli cells. We engineered an error-prone transcription sequence (A9 run) in the gene encoding the lac repressor and show that this ‘slippery’ sequence directly increases epigenetic switching, not mutation in the cell population. Therefore, one altered transcript within a multi-generational series of many error-free transcripts can cause long-term phenotypic consequences. Thus, like DNA mutations, transcriptional epimutations can instigate heritable changes that increase phenotypic diversity, which drives both evolution and disease. | 23825966
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Related Products & Applications
Categories
| Life Science Research > Cell Culture and Systems > Cell Culture Equipment > Microfluidic Cell Culture and Analysis Platform > CellASIC ONIX Microfluidic System and Accessories |
Dynamic Cell Culture and Environmental Control
The CellASIC™ ONIX Microfluidic Platform delivers breakthrough control for live cell analysis experiments by combining the highest precision controls, maximum functionality, and simplified user operation for demanding long-term perfusion experiments. The system enables dynamic time-lapse experiments never before possible. Cutting edge microfluidics technology provides an improved cell culture microenvironment, exceptional quality for high magnification microscopy, and superior media switching capabilities.
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Bio-inspired Microfluidic Plates
Our microfluidic experiment plates deliver unprecedented quality, innovation, and functionality for live cell analysis. Our plates are built with application specific design and industry leading microfluidics technology, making it easy to view multiple microchambers in parallel.
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Flexible to Your Research Needs
Intuitive and easy-to-program CellASIC™ ONIX FG Software automates your entire customizable culture protocol, so you can spend more time exploring the countless experimental possibilities enabled by this single platform.
Popular Applications of CellASIC™ Technology:
What you’ve always imagined can now be reality, using the CellASIC™ ONIX System to design dynamic cell biology experiments. It’s been demonstrated by our own scientists and loyal customers. The applications listed below are just a few of the exciting experiments you can perform with unprecedented precision:
- Cell response over time
- Interrogating 3D cell culture
- Chemotaxis/migration
- Drug dose/response
- Neural stem cell analysis
- Host-Pathogen interactions
- Hypoxic conditions to mimic tumor environments
- Bacteria single cell analysis
- Yeast single cell analysis
Features & Benefits
- Preprogram dynamic inputs to the environment, including media, activators, inhibitors, detection reagents, gas mixture, and temperature for completely hands-free operation
- Software-driven flow switching with completely customizable flow rates that can change at preset time points
- Compatible with most inverted microscopes, enabling dynamic, live-cell microscopy experiments that cannot be done in static culture dishes
- Multiple application-specific plates enable a range of experimental designs
- Easy software setup using application-specific wizards to get you started immediately
Applications
3D Cell Culture, Chemotaxis/Migration in Reponse to Chemogradient, Cell Response to Changing Media Conditions, Neural Stem Cell Analysis, Host-pathogen Interations, Bacteria Single Cell Response, Yeast Single Cell Response, Hypoxic Conditions to Mimic Tumor Environments
Product Performance
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