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219461 Cathepsin K, His•Tag®, Human, Recombinant, E. coli

Cathepsin K, His•Tag®, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.

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219461
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Overview

Replacement Information

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Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
219461-25UG
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      		 Plastic ampoule 25 μg
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      Description
      OverviewCathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His•Tag® sequence.
      Note: 1 mU = 1 milliunit.
      Catalogue Number219461
      Brand Family Calbiochem®
      References
      ReferencesDelaisse, J.M., et al. 2003. Microsc. Res. Tech. 61, 504.
      Dodds, R.A. 2003. Cell Biochem. Funct. 21, 231.
      Everts, V., et al. 2003. Calcif. Tissue Int. 73, 380.
      Hou, W.S., et al. 2003. Biol. Chem. 384, 891.
      Product Information
      CAS number94716-09-3
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.
      EC number3.4.22.38
      FormLiquid
      FormulationIn 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥900 mU/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      219461-25UG 04055977218442

      Documentation

      Cathepsin K, His•Tag®, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Cathepsin K, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      219461

      References

      Reference overview
      Delaisse, J.M., et al. 2003. Microsc. Res. Tech. 61, 504.
      Dodds, R.A. 2003. Cell Biochem. Funct. 21, 231.
      Everts, V., et al. 2003. Calcif. Tissue Int. 73, 380.
      Hou, W.S., et al. 2003. Biol. Chem. 384, 891.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-March-2013 JSW
      DescriptionCathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts. Recently reported to also be expressed in fibroblasts. Irregularities in activity contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. Contains a C-terminal His•Tag®.
      FormLiquid
      FormulationIn 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.
      Concentration Label Please refer to vial label for lot-specific concentration
      CAS number94716-09-3
      EC number3.4.22.38
      Purity≥95% by SDS-PAGE
      Specific activity≥900 mU/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesDelaisse, J.M., et al. 2003. Microsc. Res. Tech. 61, 504.
      Dodds, R.A. 2003. Cell Biochem. Funct. 21, 231.
      Everts, V., et al. 2003. Calcif. Tissue Int. 73, 380.
      Hou, W.S., et al. 2003. Biol. Chem. 384, 891.