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SCT047 BioTracker Cystine-FITC Live Cell Dye

Live cell imaging dye that measures cystine uptake at the single cell level used to detect glutathione (GSH) synthesis, reactive oxygen species (ROS) detoxification, T-cell activation and B-cell development.

BioTracker Cystine-FITC Live Cell Dye MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Based Assays
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SCT047
1 mg  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      Cell Based Assays
      Description
      Catalogue NumberSCT047
      DescriptionBioTracker Cystine-FITC Live Cell Dye
      OverviewUptake of cystine allows accumulation of cysteine that is necessary for glutathione synthesis and reactive oxygen species (ROS) detoxification. After the uptake of cystine into the cytoplasm, the reducing intracellular environment leads to a separation of one cystine molecule into two cysteine molecules. A key function of cysteine is to maintain the cellular pools of glutathione (GSH). Cystine uptake is also required for T-cell activation and B-cell development.

      The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods.

      Spectral Properties
      Absorbance: 450nm
      Emission: 525nm

      References
      Rathmell JC et al. Fluorescence-based measurement of cystine uptake through xCT shows requirement for ROS detoxification in activated lymphocytes. J Immunol Methods. 2016 Nov;438:51-58.
      Alternate Names
      • Live cell imaging probe
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized
      Applications
      ApplicationLive cell imaging dye that measures cystine uptake at the single cell level used to detect glutathione (GSH) synthesis, reactive oxygen species (ROS) detoxification, T-cell activation and B-cell development.
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
      Molar Mass: 625.65 g/mol
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker Cystine-FITC Live Cell Dye at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT047 04054839532412