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SCT281 BioTracker™ Blue Live Cell Cycle Assay Solution

Blue fluorescence probe for analysis of the cell cycle by flow cytometry.

SCT281
50 tests  
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      Overview

      Description
      Catalogue NumberSCT281
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      DescriptionBioTracker™ Blue Live Cell Cycle Assay Solution
      OverviewPropidium iodide (PI) is often used for cell cycle analysis by flow cytometry. Compared to this analysis method, the BioTracker™ Far Red Live Cell Cycle Assay Solution does not require cell fixation or permeabilization, and is permeable in live cells.

      Use 5 uL of probe solution in 500 uL for each sample. Vial contains enough for 50 tests.

      IMPORTANT APPLICATION NOTES:
      *Wash cells thoroughly to remove trypsin. Residual trypsin can confound analysis of flow cytometry populations.

      *Adjust gates after cells are stained with probe

      SPECTRAL PROPERTIES

      Excitation: 405 - 407 nm
      Emission: 450/50 BP
      Use violet laser for excitation.
      Alternate Names
      • cell cycle probe
      • cell cycle dye
      • cell cycle indicator
      • live cell probe
      Background InformationThe control mechanism of the cell cycle is closely linked to cell proliferation. The cell cycle includes cell division, in which one cell produces two daughter cells. The cell cycle can be assessed by detecting cells stained for DNA using flow cytometry, followed by analysis of the proportion of stained nuclei in cell cycle phase. The cell cycle is classified into two major phases: interphase and the mitotic (M) phase. Interphase is composed of G1 phase (preparation for DNA synthesis which occurs prior to cell division), S phase (DNA synthesis and replication), and G2 phase (chromosome duplication). M phase involves mitosis and cytokinesis. Cell cycle progression is regulated by cyclins and cyclin-dependent kinases. To determine the action of drugs such as anticancer therapeutics, it is essential to analyze the mechanism of cell-cycle checkpoint.
      Product Information
      Detection methodFlow cytometry Fluorescence
      FormatLiquid Reagent
      PresentationLiquid reagent. Aqueous solution.
      Quality LevelMQ100
      Applications
      ApplicationBlue fluorescence probe for analysis of the cell cycle by flow cytometry.
      Key Applications
      • Cell Based Assays
      • Fluorescence
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting (FACS)
      Application NotesLive cell probe for fluorescent cell cycle analysis. Unlike propidium iodide (PI), this probe is membrane permeable and highly specific for DNA, which makes it possible to analyze the cell cycle by simply adding the reagent to a cell suspension without any fixation, permeabilization, or other sample prep.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker™ Blue Live Cell Cycle Assay Solution at -20 °C, protected from light.
      Packaging Information
      Material Size50 tests
      Global Trade Item Number
      Catalogue Number GTIN
      SCT281 04065269218593