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SCCAR10065-1VL Assay Ready Mouse HL-1 Cardiac Muscle Cell Line

HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels. **SCCAR10065 cells are n

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      Overview

      Description
      Catalogue NumberSCCAR10065-1VL
      DescriptionAssay Ready Mouse HL-1 Cardiac Muscle Cell Line
      OverviewThis cell line may not be expanded. It is provided at a cell density that provides sufficient cells for a 96-well plate assay upon recovery.
      Alternate Names
      • HL-1 Cardiomyocyte Cell Line
      • HL-1 Atrial Muscle Cell Line
      • HL1 Cells
      Background InformationThis product contains cells sold under a consumable label license and which may not be propagated. For the equivalent expandable, bankable LX-2 cell line, please see SCC065.

      HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.

      The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.

      References:
      1. Claycomb, W.C., Lanson, N.A. Jr., Stallworth, B.S., Egeland, D.B., Delcarpio, J.B., Bahinski, A., Izzo, N.J. Jr. (1998). HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte. Proc. Natl. Acad. Sci. USA. 95(6): 2979-2984.
      2. White, S. M., Constantin, P. E., & Claycomb, W. C. (2004). Cardiac physiology at the cellular level: use of cultured HL-1 cardiomyocytes for studies of cardiac muscle cell structure and function. American Journal of Physiology-Heart and Circulatory Physiology, 286(3), H823-H829.
      Product Information
      Components
      • 1 vial HL-1 mouse cardiac muscle cells (Catalog No. SCCAR10065-1VL). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationHL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels. **SCCAR10065 cells are non-expandable and packaged at high density to enable readiness for hepatic fibrosis and other relevant assays without propagation or passage.
      Key Applications
      • Cell Culture
      • Cell Based Assays
      Application NotesThis product is intended for sale as a consumable. It may not be propagated, passaged, or expanded and is subject to the terms of the “Assay Ready Cells Research Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      Cell Line Type
      • Cardiomyocyte Cells
      Product Usage Statements
      Quality Assurance• Cells tested negative for infectious diseases by the Mouse Essential CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      • Functional Assay: Fluo-8 calcium dye fluorescent based assay for detecting changes in intracellular calcium
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsHL-1 Cardiac Muscle Cell Line should be stored in liquid nitrogen.
      Packaging Information
      Material Size1 vial
      Global Trade Item Number
      Catalogue Number GTIN
      SCCAR10065-1VL 04065271946293