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ALP30 Apolipoprotein B, human

Apolipoprotein B, human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Positive Control UniProt Number: P04114 Entrez Gene Number: NM_000384.2
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ALP30
500 µg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberALP30
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionApolipoprotein B, human
      OverviewTwo forms of Apo B exist: Apo B-100 and Apo B-48. The first is found in VLDL and LDL and is produced by the liver. The second is found in chylomicrons and originates in the intestine.

      Apo B is purified from LDL isolated by density gradient ultracentrifugation (1.03-1.05 g/mL density range) followed by size exclusion chromatography in 10 mM deoxycholate, 50 mM NaCl, 50 mM Na2CO3, pH 10 (Walsh & Atkinson 1983).

      PROPERTIES:

      Apo B, which has a Mr of 550 kD, is the major protein found in LDL and is responsible for the cellular uptake of LDL particles from the plasma (Goldstein & Brown 1982). The Apo B prepared by this procedure is aggregated. The aggregates are not retained by a 0.45 mm filter, but migrate only a short distance into a 5% SDS PAGE gel. In the isolation buffer, Apo B gives a stable solution that is slightly hazy.
      Alternate Names
      • ApoB
      References
      Product Information
      PresentationLyophilized from 50 mM Na2CO3, pH 10, 50 mM NaCl, and 10 mM sodium deoxycholate solution. Non-sterile.
      Quality LevelMQ100
      Applications
      Key Applications
      • Positive Control
      Biological Information
      Purity>95% by SDS-PAGE; Single arc by IEP against antisera to whole human serum.
      SourceHuman plasma, fresh, non-frozen. Tested negative for anti-HIV-1, anti-HIV-2, anti-HCV, anti-HBc and HBsAg.
      Entrez Gene Number
      Entrez Gene SummaryThis gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination.
      Gene Symbol
      • APOB
      • apoB-48
      • FLDB
      • apoB-100
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04114 # Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
      SIZE: 4563 amino acids; 515563 Da
      SUBCELLULAR LOCATION: Secreted.
      PTM: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
      DISEASE: SwissProt: P04114 # Defects in APOB are a cause of familial hypobetalipoproteinemia (FHBL) [MIM:107730]. FHBL is a genetically heterogeneous autosomal co-dominant disorder, associated with reduced plasma concentrations of apoB, LDL and VLDL. Heterozygotes for FHBL are usually asymptomatic with LDL cholesterol and apoB- 100 concentrations less than 50% of those in normal plasma. Homozygotes have extremely low plasma LDL cholesterol and apoB-100 concentrations, and clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia [MIM:200100]. & Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]. FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. & Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.SIMILARITY:SwissProt: P04114 ## Contains 1 vitellogenin domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at <= -20°C for up to six months. Reconstitute to 1 mL by adding deionized water. If the deoxycholate is removed, Apo B-100 will slowly aggregate and become insoluble. After reconstitution maintain at +2-8°C for up to 4 weeks.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ALP30 04053252474651

      Documentation

      Apolipoprotein B, human SDS

      Title

      Safety Data Sheet (SDS) 

      Apolipoprotein B, human Certificates of Analysis

      TitleLot Number
      HUMAN APOLIPOPROTEIN B 3140035
      HUMAN APOLIPOPROTEIN B 3106557
      HUMAN APOLIPOPROTEIN B 2900875
      HUMAN APOLIPOPROTEIN B 2896418
      HUMAN APOLIPOPROTEIN B 3584422
      HUMAN APOLIPOPROTEIN B - 2435083 2435083
      HUMAN APOLIPOPROTEIN B - 2246756 2246756
      HUMAN APOLIPOPROTEIN B - 3030273 3030273
      HUMAN APOLIPOPROTEIN B - 3061235 3061235
      HUMAN APOLIPOPROTEIN B - 3188658 3188658
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