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ABE1392 Anti-phospho SIRT1 (Ser682) Antibody

Anti-phospho SIRT1 (Ser682) Antibody, Cat. No. ABE1392, is a highly specific rabbit Polyclonal antibody, that targets SIRT1 and has been tested in Western Blotting.

Anti-phospho SIRT1 (Ser682) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: SIRT1, SIR2L1 UniProt Number: Q96EB6
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ABE1392
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1392
      DescriptionAnti-phospho SIRT1 (Ser682) Antibody
      Alternate Names
      • NAD-dependent protein deacetylase sirtuin-1
      • hSIRT1
      • Regulatory protein SIR2 homolog 1
      • SIR2-like protein 1
      • hSIR2
      • SirtT1 75 kDa fragment
      • 75SirT1
      • phospho SIRT1 (Ser682)
      Background InformationNAD-dependent protein deacetylase sirtuin-1 (UniProt Q96EB6; also known as hSIR2, hSIRT1, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha, Sirtuin type 1) is encoded by the SIRT1 (also known as SIR2L1) gene (Gene ID 23411) in human. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms studied. Sirtuins have been implicated in the regulation of multiple processes, including aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) and aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 and pS47 targeted by CaMKKβ.
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho SIRT1 (Ser682) Antibody, Cat. No. ABE1392, is a highly specific rabbit Polyclonal antibody, that targets SIRT1 and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phospho SIRT1 (Ser682) near the C-terminus.
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificitySer682 of hSIRT1 isoform 1 is equivalent to Ser496 of hSIRT1 spliced isoform 2 (delta-exon8).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Porcine, Feline, Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SIRT1
      • SIR2L1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~110 kDa observed. The target band size appears larger than the calculated molecular weight (81.7 kDa/isoform 1 & 61.1 kDa/isoform 2). Uncharacterized band(s) may appear in some lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Nocodazole treated H1299 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho SIRT1 (Ser682) in 10 µg of Nocodazole treated cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1392 04055977176452