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MABE229 Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

Anti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: CDK1, CDC2, CDC28A, CDKN1, P34CDC2 UniProt Number: P06493
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MABE229
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE229
      DescriptionAnti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2
      Alternate Names
      • Cyclin-dependent kinase 1
      • CDK1
      • Cell division control protein 2 homolog
      • Cell division protein kinase 1
      • p34 protein kinase
      Background InformationCyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer.
      References
      Product Information
      FormatPurified
      Control
      • UV treated and untreated HeLa cell lysates
      PresentationPurified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenLinear peptide corresponding to human Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.
      EpitopePhosphorylated Thr14 and phosphorylated Tyr15
      CloneCP3.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDK1
      • CDC2
      • CDC28A
      • CDKN1
      • P34CDC2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~34 kDa observed. An uncharacterized band may be observed at ~62 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in UV treated and untreated HeLa cell lysates.

      Western Blot Analysis: A 1:1,000 dilution from a representative lot detected phospho-Cdk1 (Thr14, Tyr15) in UV treated HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE229 04053252694752

      Documentation

      Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2 Certificates of Analysis

      TitleLot Number
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 2435676 2435676
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 2494271 2494271
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 3252817 3252817
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 3514952 3514952
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 3915638 3915638
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 4113061 4113061
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - Q2040038 Q2040038
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 -2523880 2523880
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 -2763835 2763835
      Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 Monoclonal Antibody 2924635
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies