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MABN832 Anti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody

Anti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody is an antibody against AMPA receptor for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, Enzyme Immunoassay (ELISA), Electron Microscopy and Immunofluorescence.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: GRIA1, GLUH1, GLUR1 UniProt Number: P42261, P42262, P42263, P48058
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MABN832
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IHC, ELISA, ICC, EM, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN832
      DescriptionAnti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody
      Alternate Names
      • Glutamate receptor 1
      • GluR-1
      • AMPA-selective glutamate receptor 1
      • GluR-A
      • GluR-K1
      • Glutamate receptor ionotropic
      • AMPA 1
      • GluA1
      Background InformationGlutamate receptors are synaptic receptors located primarily on the membranes of neuronal cells. Upon ligand stimulation, ionotropic glutamate receptors (iGluRs) form non-selective cation channels, while metabotropic glutamate receptors (mGluRs) indirectly activate ion channels via G protein-mediated signaling. AMPA receptors are iGluRs and are also known as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors, AMPARs, or quisqualate receptors. AMPARs are composed of four types of subunits, GluR1/GluA1 (UniProt P42261) is encoded by GRIA1, GluR2/GluA2 (UniProt P42262) by GRIA2, GluR3/GluA3 (UniProt P42263) by GRIA3, and GluR4/GluA4 (UniProt P48058) by GRIA4 gene in human. AMPARs are tetrameric in nature, consisting of two sets of homodimers, most often a set of GluR2 dimer is coupled with a set of GluR1, GluR3 or GluR4 homodimer. Cat. No. Anti-pan-AMPA receptor (GluR1-4), clone 2D8, reacts with all four AMPA receptor subunits.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody is an antibody against AMPA receptor for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, Enzyme Immunoassay (ELISA), Electron Microscopy and Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Enzyme Immunoassay (ELISA)
      • Immunocytochemistry
      • Electron Microscopy
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of human brain tissue lysate.
      Western Blotting Analysis: 1.0 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of transfected human GluA1 HEK293, transfected rat GluA1 HEK293, transfected rat GluA2 HEK293, transfected rat GluA3 HEK293, and Mock transfected cell lysate.
      Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected AMPA receptor (GluR1-4) in human cerebral cortex, human thalamus, rat cerebellum, and rat cerebral cortex tissue.
      Immunocytochemistry Analysis: A representative lot detected AMPA receptor (GluR1-4) in Hela cells transfected with GluA1, GluA2, GluA3 and GluA4 (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
      Immunofluorescence Analysis: A representative lot detected AMPA receptor (GluR1-4) in rodent, primate and human brain sections (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
      Electron Microscopy Analysis: A representative lot detected AMPA receptor (GluR1-4) in rat hippocampus (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
      ELISA Analysis: A representative lot detected AMPA receptor (GluR1-4) in TrpE fusion proteins (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
      Biological Information
      ImmunogenFusion protein coding for the extracellular domain of AMPA Glutamate receptor 1-4 corresponding to human pan-AMPA receptor (GluR1-4).
      Clone2D8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GRIA1
      • GLUH1
      • GLUR1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~85 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected AMPA receptor (GluR1-4) in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN832 04055977159332

      Documentation

      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody Certificates of Analysis

      TitleLot Number
      Anti-pan-AMPA receptor (GluR1-4), -2802547 2802547
      Anti-pan-AMPA receptor (GluR1-4), -Q2564150 Q2564150
      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 - 3153215 3153215
      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 - 35014223501422 3501422
      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 - 3587086 3587086
      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 - 3792021 3792021
      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 - 3887099 3887099
      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 - 4028575 4028575
      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 - 4101379 4101379
      Anti-pan-AMPA receptor (GluR1-4), clone 2D8 - 4144853 4144853
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      Technical Info

      Title
      White Paper: Further considerations of antibody validation and usage.