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MABE1808-25UG Anti-p53 Antibody, clone POE316A/E9

Anti-p53, clone POE316A/E9, Cat. No. MABE1808, is a highly specific rat monoclonal antibody that targets Tumor suppressor p53 and has been tested in Immunohistochemistry (Paraffin).

MABE1808-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1808-25UG
      DescriptionAnti-p53 Antibody, clone POE316A/E9
      Alternate Names
      • Cellular tumor antigen p53
      • Tumor suppressor p53
      Background InformationCellular tumor antigen p53 (UniProt: P02340; also known as Tumor suppressor p53) is encoded by the Tp53 (also known as P53, Trp53) gene (Gene ID: 22059) in murine species. p53, a well-conserved phosphoprotein, is one of the best known tumor suppressors. Human p53 consists of 393 amino acids assembled into five structurally and functionally different domains. It acts as a sequence-specific nuclear transcription factor that binds to defined consensus sites within DNA as a tetramer and represses transcription of a set of genes involved in cell growth stimulation, while activating a different set of genes involved in cell cycle control. It causes growth arrest before either DNA replication thereby providing a window for DNA repair or elimination of cells with severely damaged DNA strands. In some cell types, however, p53 activation results in apoptosis as a means of eliminating severely damaged cells. In normal, non-activated cells, the p53 signaling network is not active. However, p53 signaling is activated in cells as a response to various signals that take place during the carcinogenic process. Carcinogen-induced DNA damage, abnormal proliferative signals, hypoxia, and loss of cell adhesion are some of the most common signals that activate p53. The final outcome of p53 activation depends on many factors, and is mediated largely through the action of downstream effector genes transactivated by p53. In unstressed cells, p53 is latent and is maintained at low levels by targeted, ubiquitin-mediated degradation mediated by MDM2 and many other ubiquitin ligases. MDM2 functions as an E3 ligase to ubiquitinate p53 and force its export from the nucleus to the cytoplasm, where p53 is degraded by the proteasome.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-p53, clone POE316A/E9, Cat. No. MABE1808, is a highly specific rat monoclonal antibody that targets Tumor suppressor p53 and has been tested in Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A representative lot detected p53 in Immunohistochemistry applications (Bejarano, L., et. al. (2017). Cancer Cell. 32(5):590-607).
      Biological Information
      ImmunogenGST/His-tagged full-length recombinant mouse p53 protein.
      ClonePOE316A/E9
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone POE316A/E9 is a rat monoclonal antibody that detects murine p53.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tp53
      • P53
      • Trp53
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight43.46 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected p53 in mouse colon tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1808-25UG 04054839961823

      Documentation

      Anti-p53 Antibody, clone POE316A/E9 Certificates of Analysis

      TitleLot Number
      Anti-p53, clone POE316A/E9 - 3217465 3217465

      References

      Reference overviewPub Med ID
      Natural Killer Cells Control Tumor Growth by Sensing a Growth Factor.
      Barrow, AD; Edeling, MA; Trifonov, V; Luo, J; Goyal, P; Bohl, B; Bando, JK; Kim, AH; Walker, J; Andahazy, M; Bugatti, M; Melocchi, L; Vermi, W; Fremont, DH; Cox, S; Cella, M; Schmedt, C; Colonna, M
      Cell  172  534-548.e19  2018

      Show Abstract       		29136505 29136505