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NA35 Anti-p27kip-1 (Ab-2) Mouse mAb (DCS72)

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NA35
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~27 kDa p27kip-1 protein in HeLa cells and normal breast tissue.
      Catalogue NumberNA35
      Brand Family Calbiochem®
      References
      ReferencesKawamata, N., et al. 1995 Cancer Res. 55, 2266.
      Pietenpol, J.A., et al. 1995 Canere Res. 55, 1206.
      Ponce-Casteneda, M.V., et al. 1995 Cancer Res. 55, 1211.
      Polyak, K., et al. 1994 Genes Dev. 8, 9.
      Toyoshima, H. and Hunter, T. 1994 Cell 78, 67.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 2% gelatin, pH 7.5.
      Negative controlMOPC21 cells
      Positive controlHeLa cells or normal breast tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Cordon-Cardo, C., et al. 1998. J. Natl. Cancer Inst. 90, 1284.
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
      Paraffin Sections (1 µg/ml, heat pre-treatment required; see application references)
      Application CommentsFor staining paraffin sections, tissue should be boiled in 0.01% citric acid in a microwave for 15 min and allowed to cool prior to staining (see application references). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant p27Kip-1
      ImmunogenHuman and Mouse
      CloneDCS72
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NA35 0

      Documentation

      Anti-p27kip-1 (Ab-2) Mouse mAb (DCS72) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p27kip-1 (Ab-2) Mouse mAb (DCS72) Certificates of Analysis

      TitleLot Number
      NA35

      References

      Reference overview
      Kawamata, N., et al. 1995 Cancer Res. 55, 2266.
      Pietenpol, J.A., et al. 1995 Canere Res. 55, 1206.
      Ponce-Casteneda, M.V., et al. 1995 Cancer Res. 55, 1211.
      Polyak, K., et al. 1994 Genes Dev. 8, 9.
      Toyoshima, H. and Hunter, T. 1994 Cell 78, 67.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
      Paraffin Sections (1 µg/ml, heat pre-treatment required; see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~27 kDa p27kip-1 protein.
      Backgroundp27, the product of the p27Kip1 gene, is an inducible inhibitor of cyclin dependent kinase activity. Related to p21WAF, p27 interacts strongly with D-type cyclins in complex with cdk4 and more weakly with cyclin E and cdk2 complexes2. Based on results from experiments in which p27 is overexpressed in cell lines, it appears that p27 acts as a negative regulator of G1 progression, and in fact, increased levels of the protein have been observed in cells undergoing G1 arrest following treatment with TGFβ. The p27 gene has been localized to chromosome 12p13.
      HostMouse
      Immunogen speciesHuman and Mouse
      Immunogenrecombinant p27Kip-1
      CloneDCS72
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlHeLa cells or normal breast tissue
      Negative controlMOPC21 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor staining paraffin sections, tissue should be boiled in 0.01% citric acid in a microwave for 15 min and allowed to cool prior to staining (see application references). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesKawamata, N., et al. 1995 Cancer Res. 55, 2266.
      Pietenpol, J.A., et al. 1995 Canere Res. 55, 1206.
      Ponce-Casteneda, M.V., et al. 1995 Cancer Res. 55, 1211.
      Polyak, K., et al. 1994 Genes Dev. 8, 9.
      Toyoshima, H. and Hunter, T. 1994 Cell 78, 67.
      Application referencesParaffin Sections Cordon-Cardo, C., et al. 1998. J. Natl. Cancer Inst. 90, 1284.