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07-369-I Anti-dimethyl Histone H3 Antibody (Lys36)

Anti-dimethyl Histone H3 Antibody (Lys36) is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting, Dot Blot & Chromatin IP (ChIP).

Anti-dimethyl Histone H3 Antibody (Lys36) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
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07-369-I
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, DB, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-369-I
      DescriptionAnti-dimethyl Histone H3 Antibody (Lys36)
      Alternate Names
      • Histone H3.1t
      • H3/t/ H3t/ H3/g
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-dimethyl Histone H3 Antibody (Lys36) is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting, Dot Blot & Chromatin IP (ChIP).
      Key Applications
      • Western Blotting
      • Dot Blot
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesDot Blot Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H3 (Lys36), but not unmethylated Histone H3 or other peptides corresponding to modified histones.

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot imunoprecipitated dimethyl Histone H3 (Lys36) in HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human dimethyl Histone H3 methylated at Lys36.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to react with Vertebrates based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected dimethyl Histone H3 (Lys36) in 10 µg HeLa acid extract and demonstrated a loss of signal in 0.5 µg of unmethylated recombinant Histone H3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-369-I 04053252918643

      Documentation

      Anti-dimethyl Histone H3 Antibody (Lys36) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-dimethyl Histone H3 Antibody (Lys36) Certificates of Analysis

      TitleLot Number
      Anti-dimethyl Histone H3 (Lys36) 3072328
      Anti-dimethyl Histone H3 (Lys36) - 3193855 3193855
      Anti-dimethyl Histone H3 (Lys36) - 3425572 3425572
      Anti-dimethyl Histone H3 (Lys36) - 3856698 3856698
      Anti-dimethyl Histone H3 (Lys36) - 4131166 4131166
      Anti-dimethyl Histone H3 (Lys36) -2662263 2662263
      Anti-dimethyl Histone H3 (Lys36) -2687784 2687784
      Anti-dimethyl Histone H3 (Lys36) -2760521 2760521
      Anti-dimethyl Histone H3 (Lys36) -2794506 2794506
      Anti-dimethyl Histone H3 (Lys36) Polyclonal Antibody 2900041
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