Millipore Sigma Vibrant Logo

PC34 Anti-c-Kit (961-976) Rabbit pAb

This Anti-c-Kit (961-976) Rabbit pAb is validated for use in Immunofluorescence, Paraffin Sections, Immunoblotting for the detection of c-Kit (961-976).

Anti-c-Kit (961-976) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
PC34
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~145 kDa c-Kit protein.
      Catalogue NumberPC34
      Brand Family Calbiochem®
      References
      ReferencesLerner, N.B., et al. 1991. Blood 77, 1876.
      Tsai, M., et al. 1991. J. Exp. Med. 174, 125.
      Flanagan, J.G., and Leder, P., 1990. Cell 63, 185.
      Chabot, B., et al. 1988. Nature 335, 88.
      Geissler, E.N., et al. 1988. Cell 55, 185.
      Majumder, S., et al. 1988. Mol. Cell. Biol. 8, 4896.
      Qui, F., et al. 1988. EMBO J. 7, 1003.
      Yarden, Y., et al. 1987. EMBO J. 6, 3341.
      Besmer, P., et al. 1986. Nature 320, 415.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Negative controlHuman fibroblast cells or normal rabbit IgG
      Positive controlA172 or bone marrow cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Cattoretti, et al. 1993. J. Pathol. 171, 83.
      Key Applications Immunofluorescence
      Not Western Blot
      Paraffin Sections
      Application NotesImmunofluorescence (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, urea pre-treatment required, see comments and application references)
      Immunoblotting (not recommended)
      Application CommentsFor staining paraffin sections, pre-treat samples with 6 M urea at 95°C, 10 min. Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogena synthetic peptide (GSTASSSQPLLVHDDV) corresponding to amino acids 961-976 of human c-Kit
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC34 0

      Documentation

      Anti-c-Kit (961-976) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC34

      References

      Reference overview
      Lerner, N.B., et al. 1991. Blood 77, 1876.
      Tsai, M., et al. 1991. J. Exp. Med. 174, 125.
      Flanagan, J.G., and Leder, P., 1990. Cell 63, 185.
      Chabot, B., et al. 1988. Nature 335, 88.
      Geissler, E.N., et al. 1988. Cell 55, 185.
      Majumder, S., et al. 1988. Mol. Cell. Biol. 8, 4896.
      Qui, F., et al. 1988. EMBO J. 7, 1003.
      Yarden, Y., et al. 1987. EMBO J. 6, 3341.
      Besmer, P., et al. 1986. Nature 320, 415.

      Brochure

      Title
      Stem Cell Resource Brochure GBP
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-February-2008 JSW
      ApplicationImmunofluorescence (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, urea pre-treatment required, see comments and application references)
      Immunoblotting (not recommended)
      DescriptionProtein-A affinity purified rabbit polyclonal antibody. Recognizes the ~145 kDa c-Kit protein.
      BackgroundThe c-kit proto-oncogene has been recently identified as a member of the receptor-tyrosine kinase family and more specifically a member of the platelet derived growth factor receptor (PDGF-R) family. The c-Kit protein, the normal cellular homolog of the HZ4-feline sarcoma virus v-Kit, encodes a transmembrane receptor with a molecular weight of ~145-160 kDa. c-Kit has also been shown to be identical with the product of the W locus in mice and as such is integral to the development of mast cells and hematopoiesis. More recently, the ligand of the c-kit receptor (KL) has been identified and characterized and shown to be encoded at the murine steel (SI) locus.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (GSTASSSQPLLVHDDV) corresponding to amino acids 961-976 of human c-Kit
      IsotypeIgG
      Specieshuman
      Positive controlA172 or bone marrow cells
      Negative controlHuman fibroblast cells or normal rabbit IgG
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor staining paraffin sections, pre-treat samples with 6 M urea at 95°C, 10 min. Antibody should be titrated for optimal results in individual sample types.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesLerner, N.B., et al. 1991. Blood 77, 1876.
      Tsai, M., et al. 1991. J. Exp. Med. 174, 125.
      Flanagan, J.G., and Leder, P., 1990. Cell 63, 185.
      Chabot, B., et al. 1988. Nature 335, 88.
      Geissler, E.N., et al. 1988. Cell 55, 185.
      Majumder, S., et al. 1988. Mol. Cell. Biol. 8, 4896.
      Qui, F., et al. 1988. EMBO J. 7, 1003.
      Yarden, Y., et al. 1987. EMBO J. 6, 3341.
      Besmer, P., et al. 1986. Nature 320, 415.
      Application referencesParaffin Sections Cattoretti, et al. 1993. J. Pathol. 171, 83.