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OP55 Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76)

Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP55
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~39 kDa c-Jun/AP-1 protein in proliferating cells and most tumor tissues.
      Catalogue NumberOP55
      Brand Family Calbiochem®
      Application Data
      Detection of human c-jun by immunoblotting. Sample: Whole cell lysates (20 μg) from MCF7 cells. Primary antibody: Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) (Cat. No. OP55) (2.5 μg/ml). Detection: chemiluminescence
      References
      ReferencesVolm, M., et al. 1993. Clin. Exp. Metastasis 11, 325.
      Angel, P., et al. 1988. Nature 332, 166.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Bohmann, D., et al. 1987. .Science 238, 1386.
      Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 3316.
      Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 8516.
      Cavaleri, F., et al. 1985. Virology 143, 680.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Negative controlQuiescent cells
      Positive controlGrowing cells (such as HeLa) or breast or colon tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (2.5 µg/ml)
      Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
      Application CommentsThis is the preferred c-Jun antibody for immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant jun protein
      Clone76
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP55 0

      Documentation

      Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) Certificates of Analysis

      TitleLot Number
      OP55

      References

      Reference overview
      Volm, M., et al. 1993. Clin. Exp. Metastasis 11, 325.
      Angel, P., et al. 1988. Nature 332, 166.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Bohmann, D., et al. 1987. .Science 238, 1386.
      Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 3316.
      Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 8516.
      Cavaleri, F., et al. 1985. Virology 143, 680.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      ApplicationImmunoblotting (2.5 µg/ml)
      Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
      Application Data
      Detection of human c-jun by immunoblotting. Sample: Whole cell lysates (20 μg) from MCF7 cells. Primary antibody: Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76) (Cat. No. OP55) (2.5 μg/ml). Detection: chemiluminescence
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~32-39 kDa c-Jun/AP-1 protein.
      BackgroundThe jun gene is a cell derived sequence that has been identified as the presumed oncogene of avian sarcoma virus 17, which induces fibrosarcomas in chickens. v-Jun shares homology with the DNA binding domain of yeast transcription factor GCN4. Cloning of the proto-oncogene c-Jun demonstrated that it encoded a DNA binding protein of ~39 kDa with structural and functional properties of transcription factor AP-1. The oncogene protein product of Fos can bind to c-Jun. The Fos/Jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site. In some studies, overexpression of the c-Jun protein has been correlated with increased metastatic potential.
      HostMouse
      Immunogenrecombinant jun protein
      Clone76
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlGrowing cells (such as HeLa) or breast or colon tissue
      Negative controlQuiescent cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsThis is the preferred c-Jun antibody for immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesVolm, M., et al. 1993. Clin. Exp. Metastasis 11, 325.
      Angel, P., et al. 1988. Nature 332, 166.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Bohmann, D., et al. 1987. .Science 238, 1386.
      Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 3316.
      Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 8516.
      Cavaleri, F., et al. 1985. Virology 143, 680.