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AB5421-I Anti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody

Anti-Vesicular Glutamate Transporter 3 (VGLUT3) is an antibody against Vesicular Glutamate Transporter 3 (VGLUT3) for use in Immunocytochemistry and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: Slc17a8, Vglut3 UniProt Number: Q7TSF2
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AB5421-I
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCGpSerumPolyclonal Antibody
      Description
      Catalogue NumberAB5421-I
      ReplacesAB5421
      DescriptionAnti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody
      Alternate Names
      • Vesicular glutamate transporter 3
      • Solute carrier family 17 member 8
      • VGluT3
      Background InformationVesicular glutamate transporter 3 (UniProt Q7TSF2; also known as Solute carrier family 17 member 8, VGluT3) is encoded by the Slc17a8 (also known as Vglut3) gene (Gene ID 266767) in rat species. Vesicular glutamate transporters (VGluTs) are proton-dependent carriers responsible for the upload of the major excitatory neurotransmitter glutamate into synaptic vesicles within presynaptic terminals to allow later regulated release at the synaptic cleft. Studies conducted in mice show that VGluT1 and VGluT2 are essential for vital functions, while Vglut3-knockout mice have a normal life expectancy despite being deaf and developing anxiety and discrete basal locomotor phenotypes. VGluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VgluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. In contrast, VgluT3 is found in discrete population of non-glutamatergic neurons using other transmitters than glutamate, such as cholinergic interneurons in the dorsal and ventral striatum, subpopulations of GABAergic basket cells in the cortex and hippocampus, and serotoninergic neurons, where it is involved in more subtle modulation of local transmission. Rat VGluT3 is a 12-transmembrane (a.a. 77-97, 131-151, 154-174, 183-203, 222-242, 250-270, 315-335, 354-374, 391-411, 414-434, 448-468, 482-502) protein with 7 cytoplasmic and 6 vacuolar domains, having both its N- and C-terminal tails (a.a. 1-76 and 503-585) exposed at the cytoplasmic side.
      References
      Product Information
      FormatSerum
      HS Code3002 15 90
      PresentationGuinea pig polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Vesicular Glutamate Transporter 3 (VGLUT3) is an antibody against Vesicular Glutamate Transporter 3 (VGLUT3) for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected vesicular glutamate transporter 3 (VGluT3) in PC12 rat pheochromocytoma cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal cytoplasmic domain sequence of rat vesicular glutamate transporter 3 (VGluT3).
      EpitopeC-terminal cytoplasmic domain.
      ConcentrationPlease refer to lot specific datasheet.
      HostGuinea Pig
      SpecificityThis polyclonal guinea pig antiserum was raised against a VGluT3 C-terminal cytoplasmic domain sequence conserved between rat and murine species and shares 96% homology with the corresponding human sequence.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slc17a8
      • Vglut3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~61 kDa observed. 64.76 kDa (rat), 66.15 kDa (mouse), and 64.99/59.08 kDa (human isoform 1/2) calculated. A ~130 kDa central nervous system VGluT3 isoform has also been described (Munguba, G.C., et al. (2011). Mol. Vis. 17:413-419). Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected vesicular glutamate transporter 3 (VGluT3) in 10 µg of PC12 rat pheochromocytoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5421-I 04055977350166

      Documentation

      Anti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Vesicular Glutamate Transporter -2803913 2803913
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 3212776 3212776
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 3821558 3821558
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 3921445 3921445
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 3956071 3956071
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) - 4092767 4092767
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) -2728274 2728274
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) -2768882 2768882
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) -2840400 2840400
      Anti-Vesicular Glutamate Transporter 3 (VGluT3) -Q2679613 Q2679613
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