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ABT1370 Anti-Versican Antibody, Beta Domain

Anti-Versican, Beta Domain, Cat. No. ABT1370 is a rabbit polyclonal antibody that detects versican core protein and has been tested for use in Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.

Anti-Versican Antibody, Beta Domain MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Vcan, Cspg2 UniProt Number: Q9ERB4
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ABT1370
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIH(P), RIA, WBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABT1370
      DescriptionAnti-Versican Antibody, Beta Domain
      Alternate Names
      • Chondroitin sulfate proteoglycan core protein 2
      • Glial hyaluronate-binding protein
      • GHAP
      • Large fibroblast proteoglycan
      • PG-M
      Background InformationVersican core protein (UniProt: Q9ERB4; also known as Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M) is encoded by the Vcan (also known as Cspg2) gene (Gene ID: 114122) in rat. Versican is a large multi-domain chondroitin sulphate proteoglycan that is secreted by fibroblasts and other types of cells. Versican is present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer. It is synthesized with a 20 amino acid signal peptide, which is subsequently cleaved. Veriscan plays a role in intercellular signaling and in connecting cells with the extracellular matrix and is also involved in axon regeneration, central nervous system and skeletal muscle development, and cell adhesion. It has unique N- and C-terminal globular regions, each with multiple motifs. It contains one C-type lectin domain, two EGF-like domains, one Ig-like V-type domain, two link domains and one Sushi (CCP/SCR) domains. It also has a N-terminal hyaluronic acid (HA) binding domain that has homology with the G1 and G2 domains of aggrecan. Four isoforms of versican termed as V0, V1, V2, and V3, have been reported with molecular weights of 370, 263, 180, and 74 kDa, respectively. Each isoform contains different lengths of the GAG binding region. V0 contains two GAG binding regions called CSalpha and CSbeta domains. V1 isoform has only CSbeta and V2 isoform has only CSalpha domain. V3 isoform contains only G1 and G3 domains and lacks GAG attachment sites. Defects in Vcan gene result in Wagner syndrome type 1, a type of vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a pre-retinal avascular membrane.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Versican, Beta Domain, Cat. No. ABT1370 is a rabbit polyclonal antibody that detects versican core protein and has been tested for use in Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Radioimmunoassay
      • Western Blotting
      Application NotesRadioimmunoassay Analysis: A representative lot detected Versican, Beta Domain in Postnatal rat brain (Milev, P., et. al. (1998). Biochem Biopys Res Commun. 247(2):207-12).

      Immunohistochemistry Analysis: A representative lot detected Versican, Beta Domain in Embryonic day 16 rat eye, P0, P9 and adult rat retina (Popp, S., et. al. (2004). Exp Eye Res. 79(3):351-6) and Embryonic day 16, 19 rat brain and postnatal day 7 cerebellum, embryonic day 19 spinal cord (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).

      Western Blotting Analysis: A representative lot detected Versican, Beta Domain in purified proteoglycans from a PBS extract of 7-day rat brain with chondroitinase ABC treatment (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the 375 amino acids from the internal region of rat Versican.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects versican in rat eye sections.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Vcan
      • Cspg2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight300 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat eye retina tissue.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Versican, Beta Domain in rat eye retina tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT1370 04054839240300