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ABC1434 Anti-UVRAG-FS mutant

Anti-UVRAG-FS mutant, Cat. No. ABC1434, detects FS mutant UV radiation resistance-associated gene protein. Validated for use in Immunoprecipitation and Western Blotting.

Anti-UVRAG-FS mutant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: UVRAG UniProt Number: Q9P2Y5
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ABC1434
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbSemi-PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1434
      DescriptionAnti-UVRAG-FS mutant
      Alternate Names
      • UV radiation resistance-associated gene protein
      • p63
      • UVRAG-FS
      Background InformationUV radiation resistance-associated gene protein (UniProt: Q9P2Y5; also known as p63) is encoded by UVRAG gene (Gene ID: 7405) in human. UVRAG is a key autophagic tumor suppressor that is shown to be mutated in several common human cancers. UVRAG as a critical regulator of intracellular membrane trafficking, autophagy, and chromosomal stability. It contains four functional domains, a proline-rich domain, a lipid-binding C2 domain, a Beclin1-binding coiled-coil domain (CCD) and a C-terminal domain. UVRAG is truncated by a frameshift (FS) mutation in colorectal cancer cells. UVRAG-FS is shown to abolish the normal functions of UVRAG, including autophagy, in a dominant-negative manner. Ectopic expression of UVRAG-FS in both embryonic stem cells and cancer cells results in extensive centrosome amplification and concomitant aneuploidy. UVRAG-FS can also enhance the sensitivity to anticancer agents, such as 5-fluorouracil (5-FU), oxaliplatin, and irinotecan. Expression of UVRAG-FS is reported to trigger colorectal cancer metastatic spread through Rac1 activation and epithelial-to-mesenchymal transition, independently of autophagy. UVRAG-FS metastatic tumors display higher levels of p62 than primary tumors, which indicates suppressed autophagy. (Ref.: He, S., et al. (2015). Nat Commun. 6: 7839).
      References
      Product Information
      FormatSemi-Purified
      PresentationRabbit polyclonal antibody 1XPBS with 0.05% azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-UVRAG-FS mutant, Cat. No. ABC1434, detects FS mutant UV radiation resistance-associated gene protein. Validated for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected UV radiation resistance-associated gene protein in Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines (Data courtesy of Dr Liang Laboratory).

      Western Blotting Analysis: A representative lot detected Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines. (He, S., et. al. (2015). Nat Commun. 6:7839).

      Immunoprecipitation Analysis: A representative lot detected Wild-type (WT) and FS mutant UVRAG protein expression in MSS and MSI CRC cell lines. (He, S., et. al. (2015). Nat Commun. 6:7839).
      Biological Information
      ImmunogenA frameshift (FS)-UVRAG derived linear peptide.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets an internal sequence in the N-terminal half and detects UVRAG-FS by Western blotting.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UVRAG
      Purification MethodAmmonium Sulfate
      UniProt Number
      Molecular Weight~32 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in WT/ Mutant UVRAG-FS lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UV radiation resistance-associated gene protein in Wild-type (WT) and FS mutant UVRAG protein expression in microsatellite stable (MSS) and microsatellite instable (MSI) colorectal cancer cell lines.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1434 04054839156434