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MABT205 Anti-α-Tubulin Antibody, clone DM1A

This Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin.

Anti-α-Tubulin Antibody, clone DM1A MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton UniProt Number: P02552
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MABT205
100 µg  
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      Overview

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      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, ChWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT205
      Replaces04-1117
      DescriptionAnti-α-Tubulin Antibody, clone DM1A
      Alternate Names
      • Tubulin alpha-1 chain
      Background InformationTubulin is one of the main components of the cytoskeleton. It is a heterodimeric structure consisting of interlocking alpha and beta chains. Like other cytoskeleton proteins, tubulin undergoes dynamic changes depending on the cellular context, which are mediated by binding and release of GDP/GTP. Tubulin proteins may also undergo post-translational modifications, and are essential to cellular transport and mitotic processes.
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected α-tubulin in NIH/3T3, A431, and HeLa cells.

      Immunohistochemistry Analysis: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue.
      Biological Information
      ImmunogenNative chicken brain microtubules.
      CloneDM1A
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Chicken
      Species Reactivity NoteDemonstrated to react wtih Human and Mouse. Predicted to react with Chicken based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

      SUBUNIT STRUCTURE: Dimer of alpha and beta chains.

      SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton.

      POST-TRANSLATIONAL MODIFICATION: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.

      MISCELLANEOUS: There are at least seven alpha tubulin genes (alpha-1 to alpha-6, and alpha-8), and a pseudogene (alpha-7) in chicken.

      SEQUENCE SIMILARITIES: Belongs to the tubulin family.
      Molecular Weight~60 kDa observed. Uniprot describes a molecular weight at ~46 kDA, but can be higher due to post-translational modification. This protein has been observed at ~55 kDa in some cell lysates (Saugstad, J. A., et al. (2002). J Neurochem. 80(6):980-988.).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT205 04053252549328