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MABC260 Anti-TRAF1 Antibody, clone 1F3

Anti-TRAF1, clone 1F3 detects levels of TRAF1 proteins & has been published & validated for use in WB, IHC & Flow.

Anti-TRAF1 Antibody, clone 1F3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: TRAF1, EBI6 UniProt Number: Q13077
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MABC260
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Mk, HWB, IHC, FCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC260
      DescriptionAnti-TRAF1 Antibody, clone 1F3
      Alternate Names
      • TNF receptor-associated factor 1
      • TNF receptor-associated factor 1
      Background InformationTNF receptor-associated factor 1 (UniProt: Q13077; also known as Epstein-Barr virus-induced protein 6, TRAF1) is encoded by the TRAF1 (also known as EB16) gene (Gene ID: 7185) in human. TRAF1 is homodimeric pro-survival adapter molecule that regulates the activation of NF-kB and JNK. It can also exist as a heterotrimer with TRAF2. It plays a role in the regulation of cell survival and apoptosis. The heterotrimer form is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. The C-terminal region of TRAF1 contains the TRAF domain (aa 266-412) that binds to receptor cytoplasmic domains. It also has a coiled coil domain (aa 182-264) that mediates its homo- and heterotrimerization. TRAF1 is also reported to essential for memory T cell survival. TRAF1 protein level is significantly reduced in HIV-specific CD8 T cells with progression of infection. Its levels can increase following treatment with IL-7. Hence, TRAF1 levels could serve as a useful parameter in monitoring effectiveness of anti-HIV therapies. (Ref.: Wang, C., et al. (2018). J. Immunol. 200(2); 558-564).
      References
      Product Information
      FormatPurified
      Control
      • B95-8cell lysate
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TRAF1, clone 1F3 detects levels of TRAF1 proteins & has been published & validated for use in WB, IHC & Flow.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect TRAF1 in certain cell lines (Siegler, G., et al. (2003). J Clin Pathol: Mol Pathol. 56:156-161.).
      Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect TRAF1 in PBMCs and in certain T cells from HIV-infected individuals (Wang, C., et al. (2011). JEM.1-15.).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human TRAF1.
      Clone1F3
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Monkey
      • Human
      Species Reactivity NoteDemonstrated to react with Human and Monkey. Predicted to react with Human based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRAF1
      • EBI6
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~46 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~33 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in B95-8 cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected TRAF1 in 10 µg of B95-8cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC260 04053252831409

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies