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AP1058 Anti-TOM70 Rabbit pAb

Anti-TOM70 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Translocase Outer Mitochondrial Membrane

AP1058
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~70 kDa TOM70 protein in mouse liver mitochondria.
      Catalogue NumberAP1058
      Brand Family Calbiochem®
      SynonymsAnti-Translocase Outer Mitochondrial Membrane
      Application Data
      Detection of rat TOM70 by immunoblotting: Samples: whole extract (100 µg) from rat liver mitochondria. Primary antibody: Anti-TOM70 Rabbit pAb (Cat. No. AP1058) (0.25 µg/ml). Detection: chemiluminescence. Data courtesy of Sandra Wiley and Matthew Rardin, University of California San Diego.
      References
      ReferencesLee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Suzuki, H., et al. 2002. J. Cell Sci. 9, 1895.
      Endo, T., et al. 2002. Biochim. Biophys. Acta. 1, 3.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlRat liver mitochondria
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (0.25 µg/ml)
      Application CommentsAntibody should titrated for optimal results in individual sytems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids surrounding Ser⁹⁴ of human TOM70
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1058 0

      Documentation

      Anti-TOM70 Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1058

      References

      Reference overview
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Suzuki, H., et al. 2002. J. Cell Sci. 9, 1895.
      Endo, T., et al. 2002. Biochim. Biophys. Acta. 1, 3.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-May-2008 JSW
      SynonymsAnti-Translocase Outer Mitochondrial Membrane
      ApplicationImmunoblotting (0.25 µg/ml)
      Application Data
      Detection of rat TOM70 by immunoblotting: Samples: whole extract (100 µg) from rat liver mitochondria. Primary antibody: Anti-TOM70 Rabbit pAb (Cat. No. AP1058) (0.25 µg/ml). Detection: chemiluminescence. Data courtesy of Sandra Wiley and Matthew Rardin, University of California San Diego.
      DescriptionImmunoaffinty purified rabbit polyclonal antibody. Recognizes the ~70 kDa TOM70 protein.
      BackgroundThe translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM20 and TOM70 are the two major receptor subunits of the TOM complex and constitute the binding site for mitochondrial proteins. TOM70 is anchored in the outer membrane by a hydrophobic segment near the N-terminus with the 65 kD C-terminus facing the cytosol. TOM70 forms a dimer and is associated with the TOM complex via interactions with TOM20. TOM70 binds various precursor proteins, including cytochrome c1 and aldehyde dehydrogenase III. Substrates for TOM70 are highly hydrophobic proteins and require molecular chaperones in the cytosol, such as Hsp70 and mitochondrial import stimulating factor (MSF), to maintain themselves in an import-competent state. Phosphorylation of TOM70 has been detected in screens of the mitocohondrial proteome and TOM70 had been shown to be required for proper targeting of the apopoptsis regulator Mcl-1.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids surrounding Ser⁹⁴ of human TOM70
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlRat liver mitochondria
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should titrated for optimal results in individual sytems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Suzuki, H., et al. 2002. J. Cell Sci. 9, 1895.
      Endo, T., et al. 2002. Biochim. Biophys. Acta. 1, 3.