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AP1167 Anti-TNS4 Mouse mAb (3B8)

This Anti-TNS4 Mouse mAb (3B8) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of TNS4.

Anti-TNS4 Mouse mAb (3B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tensin 4, Anti-CTEN, Anti-C Terminal Tensin Like Protein

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AP1167
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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AP1167-100UG
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      Description
      OverviewRecognizes the ~52 kDa TNS4 protein in HeLa cells.
      Catalogue NumberAP1167
      Brand Family Calbiochem®
      SynonymsAnti-Tensin 4, Anti-CTEN, Anti-C Terminal Tensin Like Protein
      Application Data
      Detection of human TNS4 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human TNS4 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-TNS4 Mouse mAb (3B8) (Cat. No. AP1167) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TNS4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TNS4 Mouse mAb (3B8) (Cat. No. AP1167) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesSakashita, K., et al. 2008. Ann Surg Oncol. 15, 2606.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant human TNS4 (aa 1-481), expressed as a GST fusion protein
      Clone3B8
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1167-100UG 04055977212686

      Documentation

      Anti-TNS4 Mouse mAb (3B8) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Sakashita, K., et al. 2008. Ann Surg Oncol. 15, 2606.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-October-2010 JLN
      SynonymsAnti-Tensin 4, Anti-CTEN, Anti-C Terminal Tensin Like Protein
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human TNS4 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human TNS4 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-TNS4 Mouse mAb (3B8) (Cat. No. AP1167) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TNS4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TNS4 Mouse mAb (3B8) (Cat. No. AP1167) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~52 kDa TNS4 protein.
      BackgroundTNS4 may be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton. It may also promote apoptosis, via its cleavage by caspase 3.
      HostMouse
      ImmunogenFull-length, recombinant human TNS4 (aa 1-481), expressed as a GST fusion protein
      Clone3B8
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesSakashita, K., et al. 2008. Ann Surg Oncol. 15, 2606.