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CB1027 Anti-TNNT2 Mouse mAb (3H4-F7)

This Anti-TNNT2 Mouse mAb (3H4-F7) is validated for use in ELISA, Immunoblotting for the detection of TNNT2.

Anti-TNNT2 Mouse mAb (3H4-F7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tropin T Type 2

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CB1027
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~37 kDa TNNT2 protein in COLO 320 HSR cells.
      Catalogue NumberCB1027
      Brand Family Calbiochem®
      SynonymsAnti-Tropin T Type 2
      Application Data
      Detection of human TNNT2 by immunoblotting. Sample: Whole cell lysate from COLO 320 HSR cells. Primary antibody: Anti-TNNT2 Mouse mAb (3H4-F7) (Cat. No. CB1027) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlCOLO 320 HSR cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-268 of human TNNT2, expressed as a GST fusion protein
      ImmunogenNA
      Clone3H4-F7
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CB1027 0

      Documentation

      Anti-TNNT2 Mouse mAb (3H4-F7) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2010 RFH
      SynonymsAnti-Tropin T Type 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human TNNT2 by immunoblotting. Sample: Whole cell lysate from COLO 320 HSR cells. Primary antibody: Anti-TNNT2 Mouse mAb (3H4-F7) (Cat. No. CB1027) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa TNNT2 protein.
      BackgroundThe protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.
      HostMouse
      Immunogen speciesNA
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-268 of human TNNT2, expressed as a GST fusion protein
      Clone3H4-F7
      IsotypeIgG₁
      Specieshuman
      Positive controlCOLO 320 HSR cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review