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MAB3216-100ug Anti-TNF Receptor Antibody, extracellular, clone H398

Detect TNF Receptor using this Anti-TNF Receptor Antibody, extracellular, clone H398 validated for use in ELISA, FC, IP, RIA & WB.

Research Category: Inflammation & Immunology Research Sub-Category: Cytokines & Cytokine Receptors Gene Symbol: TNFRSF1A, TNFR1, TNF-R1, TNFR-I, p60, CD120a, p55-R, TNF-RI, TNFR60, TNF-R55, TNFAR, TBP1, TNFR55, TNF-R-I, FPF, TNF-R, p55, MGC19588 UniProt Number: P19438
MAB3216-100ug
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, FC, IP, RIA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3216-100ug
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-TNF Receptor Antibody, extracellular, clone H398
      Alternate Names
      • CD120a
      • TNF-R1
      • p55
      Background InformationTumor necrosis factor receptor superfamily member 1A (TNF-receptor 1 or CD120a) is a receptor for TNFSF2/TNF-alpha. TNF receptor mediates apoptosis and contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain of TNF receptor leads to homotrimerization. Defects in TNF receptor cause tumor necrosis factor receptor-associated periodic syndrome (TRAPS), a hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia.
      Product Information
      FormatPurified
      PresentationIgG fraction purified from tissue culture supernatant by protein-A affinity chromatography. Liquid in PBS pH 7.4 containing 0.09% sodium azide.
      Applications
      ApplicationDetect TNF Receptor using this Anti-TNF Receptor Antibody, extracellular, clone H398 validated for use in ELISA, FC, IP, RIA & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      Application NotesELISA

      Flow Cytometry

      Western Blotting: non reducing conditions only

      Immunoprecipitation

      Radioimmunoassay

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant human tumor necrosis factor receptor type 1
      EpitopeExtracellular Domain
      CloneH398
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes an extracellular domain of the 55 kDa human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the 75 kDa TNF receptor (CD120b). This antibody inhibits the biological activity of both natural and recombinant human TNFa and TNFbthrough competitive inhibition of TNF binding to TNF-R1. MAB3216-100ug shows potent antagonistic activity in in vitro TNF mediated cytotoxicity assays (IC50=12nM; IC50=1.7 mg/mL).
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.
      Gene Symbol
      • TNFRSF1A
      • TNFR1
      • TNF-R1
      • TNFR-I
      • p60
      • CD120a
      • p55-R
      • TNF-RI
      • TNFR60
      • TNF-R55
      • TNFAR
      • TBP1
      • TNFR55
      • TNF-R-I
      • FPF
      • TNF-R
      • p55
      • MGC19588
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19438 # Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
      SIZE: 455 amino acids; 50495 Da
      SUBUNIT: Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Secreted.
      DOMAIN: SwissProt: P19438 The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE. & Both the cytoplasmic membrane-proximal region and the C- terminal region containing the death domain are involved in the interaction with TRPC4AP (By similarity).
      PTM: The soluble form is produced from the membrane form by proteolytic processing.
      DISEASE: "SwissProt: P19438 # Defects in TNFRSF1A are the cause of familial hibernian fever (FHF) [MIM:142680]; also known as tumor necrosis factor receptor-associated periodic syndrome (TRAPS). FHF is an autosomal dominant disease characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia."
      SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
      Molecular Weight~50 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for short term or -20°C up to one year.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3216-100ug 0