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04-1552 Anti-TLS Antibody, clone 1FU-1D2

Use Anti-TLS Antibody, clone 1FU-1D2 (Mouse Monoclonal Antibody) validated in WB, ICC to detect TLS also known as 75 kDa DNA-pairing protein, FUS-CHOP fusion protein.

Anti-TLS Antibody, clone 1FU-1D2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins, Transcription Factors Gene Symbol: FUS, CHOP, FUS-CHOP, FUS1, POMp75, TLS, TLS/CHOP, hnRNP-P2 UniProt Number: P35637
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04-1552
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCMAscitesMonoclonal Antibody
      Description
      Catalogue Number04-1552
      DescriptionAnti-TLS Antibody, clone 1FU-1D2
      Alternate Names
      • 75 kDa DNA-pairing protein
      • FUS-CHOP fusion protein
      • FUS-CHOP protein fusion
      • Fusion gene in myxoid liposarcoma
      • Oncogene FUS
      • Oncogene TLS
      • Translocated in liposarcoma protein
      • fus-like protein
      • fus/tls-chop oncogene
      • fusion (involved in t(12
      • 16) in malignant liposarcoma)
      • fusion, derived from t(12
      • 16) malignant liposarcoma
      • heterogeneous nuclear ribonucleoprotein P2
      • translocated in liposarcoma
      Background InformationThe TLS/FUS protein contains an RNA-recognition motif and is a component of nuclear riboprotein complexes. It resembles a transcription factor in that it binds DNA, contributes a transcriptional activation domain to the FUS-ERG oncoprotein, and interacts with several transcription factors in vitro. It binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. It may play a role in maintenance of genomic integrity.
      References
      Product Information
      FormatAscites
      Control
      • HepG2 cell lysate
      PresentationUnpurified mouse monoclonal IgG1κ preservative-free ascites.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-TLS Antibody, clone 1FU-1D2 (Mouse Monoclonal Antibody) validated in WB, ICC to detect TLS also known as 75 kDa DNA-pairing protein, FUS-CHOP fusion protein.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis:
      1:500 dilution from a representative lot detected TLS in C2C12 cells.
      Biological Information
      ImmunogenLinear peptide corresponding to human TLS.
      EpitopeUnknown
      Cloneclone 1FU-1D2
      HostMouse
      SpecificityThis antibody recognizes TLS.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq].
      Gene Symbol
      • FUS
      • CHOP
      • FUS-CHOP
      • FUS1
      • POMp75
      • TLS
      • TLS/CHOP
      • hnRNP-P2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.

      SUBUNIT STRUCTURE: Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP.

      SUBCELLULAR LOCATION: Nucleus

      TISSUE SPECIFICITY: Ubiquitous.

      PTM: Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.

      INVOLVEMENT IN DISEASE: A chromosomal aberration involving FUS is a cause of a form of malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.

      A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.

      A chromosomal aberration involving FUS is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.

      Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.

      SEQUENCE SIMILARITIES: Belongs to the RRM TET family.

      Contains 1 RanBP2-type zinc finger.

      Contains 1 RRM (RNA recognition motif) domain.
      Molecular WeightObserved at 65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HepG2 cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected TLS on 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-1552 04053252586767