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The following MAPmates™ should not be plexed together:
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AP1179
Sigma-AldrichAnti-TLR4 Mouse mAb (1H7)
This Anti-TLR4 Mouse mAb (1H7) is validated for use in ELISA, Immunoblotting for the detection of TLR4.
More>>This Anti-TLR4 Mouse mAb (1H7) is validated for use in ELISA, Immunoblotting for the detection of TLR4. Less<<
Anti-TLR4 Mouse mAb (1H7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 214-292 human TLR4, expressed as a GST fusion protein
Clone
1H7
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Anti-TLR4 Mouse mAb (1H7) Certificates of Analysis
Title
Lot Number
AP1179
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-October-2010 RFH
Synonyms
Anti-Toll-like Receptor 4
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human TLR4 by immunoblotting. Sample: Partial recombinant TLR4. Primary antibody: Anti-TLR4 Mouse mAb (1H7) (Cat. No. AP1179) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the TLR4 protein.
Background
TLR4 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 214-292 human TLR4, expressed as a GST fusion protein
Clone
1H7
Isotype
IgG2a
Species
human
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
