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GF06 Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3)

This Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) is validated for use in Immunoblotting for the detection of TGF-α (Ab-1).

Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Transforming Growth Factor-α

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, RMMonoclonal Antibody
      Description
      OverviewRecognizes the ~6 kDa TGF-α protein in A2058 and ST-FeSVFRE cells.
      Catalogue NumberGF06
      Brand Family Calbiochem®
      SynonymsAnti-Transforming Growth Factor-α
      References
      ReferencesSorvillo, J.M., McCormack, E.S. 1990. Oncogene 5, 377.
      Coffey, R.J., et al. 1987. Nature 328, 817.
      Derynck, R. 1986. J. Cell. Biochem. 32, 203.
      Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
      Lee, D.C., et al. 1985 Mol. Cell. Biol. 5, 3644.
      Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
      Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
      Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
      Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlNIH3T3 cells
      Positive controlA2058 or ST-FeSV FRE cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone, Species Reactivity Sorvillo, J.M., McCormack, E.S., 1990. Oncogene 5, 377. Immunoblotting Leong, H., et al. 2001. Carcinogenesis 22, 1809. Bush, M.R., et al. 1998. Biol. Reprod. 59, 1522.
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-5 µg/ml, see application references)
      Application CommentsThis antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human TGF-α (~6 kDa mature form)
      ImmunogenHuman
      Clone134A-2B3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GF06 0

      Documentation

      Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) Certificates of Analysis

      TitleLot Number
      GF06

      References

      Reference overview
      Sorvillo, J.M., McCormack, E.S. 1990. Oncogene 5, 377.
      Coffey, R.J., et al. 1987. Nature 328, 817.
      Derynck, R. 1986. J. Cell. Biochem. 32, 203.
      Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
      Lee, D.C., et al. 1985 Mol. Cell. Biol. 5, 3644.
      Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
      Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
      Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
      Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.

      Citations

      Title
    • Hoyee Leong, Gary L. Firestone and Leonard B. Bjeldanes. (2001) Cytostatic Effects of 3,3'-Diindolylmethane in Human Endometrial Cancer Cells Result From an Estrogen Receptor-Mediated Increase in Transforming Growth Factor-α Expression. Carcinogenesis 22, 1809-1817.
    • Mark R. Bush, et al. (1998) Evidence of Juxtacrine Signaling for Transforming Growth Factor a in Human Endometrium. 59, 1522-1529.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      SynonymsAnti-Transforming Growth Factor-α
      ApplicationImmunoblotting (1-5 µg/ml, see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa denatured and native forms of TGFα protein.
      BackgroundTransforming growth factor-alpha (TGFα) is an acid- and heat-stable 50 amino acid protein of 5500 MW originally found in rodents and humans. TGFαis 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFα binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGFα is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFα bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human TGF-α (~6 kDa mature form)
      Clone134A-2B3
      IsotypeIgG₁
      Specieshuman, rat
      Positive controlA2058 or ST-FeSV FRE cells
      Negative controlNIH3T3 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSorvillo, J.M., McCormack, E.S. 1990. Oncogene 5, 377.
      Coffey, R.J., et al. 1987. Nature 328, 817.
      Derynck, R. 1986. J. Cell. Biochem. 32, 203.
      Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
      Lee, D.C., et al. 1985 Mol. Cell. Biol. 5, 3644.
      Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
      Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
      Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
      Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
      Citation
    • Hoyee Leong, Gary L. Firestone and Leonard B. Bjeldanes. (2001) Cytostatic Effects of 3,3'-Diindolylmethane in Human Endometrial Cancer Cells Result From an Estrogen Receptor-Mediated Increase in Transforming Growth Factor-α Expression. Carcinogenesis 22, 1809-1817.
    • Mark R. Bush, et al. (1998) Evidence of Juxtacrine Signaling for Transforming Growth Factor a in Human Endometrium. 59, 1522-1529.
      		•
      Application referencesOriginal Clone, Species Reactivity Sorvillo, J.M., McCormack, E.S., 1990. Oncogene 5, 377. Immunoblotting Leong, H., et al. 2001. Carcinogenesis 22, 1809. Bush, M.R., et al. 1998. Biol. Reprod. 59, 1522.

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      Categories

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