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MABE662 Anti-TERT Antibody, clone 7D5.2

Anti-TERT, clone 7D5.2 , Cat. No. MABE662, is a highly specific mouse monoclonal antibody that targets hTERT and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-TERT Antibody, clone 7D5.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: TERT, CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TRT UniProt Number: O14746
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MABE662
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE662
      ReplacesMABE14
      DescriptionAnti-TERT Antibody, clone 7D5.2
      Alternate Names
      • Telomerase reverse transcriptase
      • hEST2
      • hTRT
      • Telomerase-associated protein 2
      • Telomerase catalytic subunit
      • TP2
      Background InformationTelomerase reverse transcriptase (EC 2.7.7.49; UniProt O14746; also known as hEST2, hTRT, Telomerase-associated protein 2, Telomerase catalytic subunit, TP2) is encoded by the TERT (also known as CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TRT) gene (Gene ID 7015) in human. Telomerase reverse transcriptase (TERT) is the catalytic subunit of the telomerase responsible for adding TTAGGG repeats to the chromosome telomere ends. Cells with low or no telomerase expression lose telomere repeats during cell division, eventually resulting in cellular senescence. Most cancer cells, germ cells and embryonic stem cells express high levels of telomerase, thus contributing to pluripotency and immortality. In addition to its telomere maintenance function, telomerase also has a pro-survival role in cellular resistance against DNA damage and ensuing apoptosis. Most cancer cells are highly proliferative and express high levels of nuclear telomerase activity. Studies show that TERT shuttles from the nucleus into mitochondria upon oxidative stress induction in cancer cells. TERT mitochondrial localization helps prevent nuclear DNA damage by decreasing mitochondrial reactive oxygen species (ROS), accounting for high stress resistance especially among the cancer stem cells (CSCs) population. Following exposure to H2O2 or gamma-irradiation, cancer cells capable of excluding TERT from the nucleus display little or no DNA damage, while TERT nuclear retainment results in high DNA damage.
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-TERT, clone 7D5.2 , Cat. No. MABE662, is a highly specific mouse monoclonal antibody that targets hTERT and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TERT in human cerebral cortex, kidney, and tonsil tissue sections.

      Western Blotting Analysis: 1 µg/mL from a representative lot detected TERT in 10 µg of Jurkat cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence toward the end of the C-terminal extension (CTE) region of human TERT.
      Clone7D5.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7D5.2 targets a sequence toward the end of the C-terminal extension (CTE) region present in spliced isoforms 1 and 3, but not in isoforms 2 and 4 of human TERT reported by UniProt (O14746).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TERT
      • CMM9
      • DKCA2
      • DKCB4
      • EST2
      • PFBMFT1
      • TCS1
      • TRT
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~140 kDa observed. 127.0/120.0 kDa (human isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC3 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected TERT in 10 µg of PC3 human prostate cancer cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABE662 04054839073496