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PC325 Anti-α-Synuclein (Ab-1) (123-140) Guinea pig pAb

This Anti-α-Synuclein (Ab-1) (123-140) Guinea pig pAb is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence for the detection of α-Synuclein (Ab-1) (123-140).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC325
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      B, H, M, RGpPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.




      Recognizes the α-synuclein protein.
      Catalogue NumberPC325
      Brand Family Calbiochem®
      References
      ReferencesTakeda, A., et al. 1998. Am. J. Pathol. 152, 367.
      Nussbaum, R.L. and Polymeropoulos, M.H., 1997. Hum. Mol. Genet. 6, 1687.
      Wakabayashi, K., et al. 1997. Neurosci. Lett. 239, 45.
      Campion, D., et al. 1995. Genomics 26, 254.
      Iwai, A., et al. 1995. Neuron 14, 467.
      Brookes, A.J. and St. Clair, D. 1994. Trends Neurosci. 17, 404.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesFrozen Sections (1:8000-1:10,000)
      Immunoblotting (1:2000, chemiluminescence)
      Immunofluorescence (1:8000-1:10,000)
      Application CommentsCan be used to localize α-synuclein in the central nervous system. For frozen sections, tissue should be fixed in 4% paraformaldehyde and 0.4% picric acid in 0.16 M sodium phosphate buffer, pH 6.9. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (EAYEMPSEEGYQDYEPEA) corresponding to amino acids 123-140 of the C-terminus of rat α-synuclein
      ImmunogenRat
      HostGuinea Pig
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC325 0

      Documentation

      Anti-α-Synuclein (Ab-1) (123-140) Guinea pig pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-α-Synuclein (Ab-1) (123-140) Guinea pig pAb Certificates of Analysis

      TitleLot Number
      PC325

      References

      Reference overview
      Takeda, A., et al. 1998. Am. J. Pathol. 152, 367.
      Nussbaum, R.L. and Polymeropoulos, M.H., 1997. Hum. Mol. Genet. 6, 1687.
      Wakabayashi, K., et al. 1997. Neurosci. Lett. 239, 45.
      Campion, D., et al. 1995. Genomics 26, 254.
      Iwai, A., et al. 1995. Neuron 14, 467.
      Brookes, A.J. and St. Clair, D. 1994. Trends Neurosci. 17, 404.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      ApplicationFrozen Sections (1:8000-1:10,000)
      Immunoblotting (1:2000, chemiluminescence)
      Immunofluorescence (1:8000-1:10,000)
      DescriptionGuinea pig polyclonal antibody supplied as undiluted serum. Recognizes the α-synuclein protein.
      BackgroundThe major component of the vascular and plaque amyloid deposits in Alzheimer's disease (AD) is the amyloid β peptide. Non-A β component of Alzheimer's disease amyloid (NAC) is the second component in the amyloid plaques from brain tissues of AD patients. Its precursor protein (NACP) is now known to be identical to α-synuclein, a presynaptic protein in the human brain. In AD, synuclein/NAC proteins are found in presynaptic cholinergic nerve terminals that degenerate early in AD and are closely linked to β amyloid fibrils in senile plaques. α-synuclein is a highly conserved protein that shows 95% homology with rat synuclein 1 and good homology with Torpedo synuclein from the electric organ synapse and bovine phosphoneuroprotein 14 (PNP-14), a brain specific protein present in synapses. Recently α-synuclein has been linked to some cases of familial Parkinson's disease (PD), localizing in Lewy bodies, hallmark lesions of degenerating neurons in the brains of patients with PD. A missense mutation in the gene encoding α-synuclein gene has been identified in some of these PD patients.
      HostGuinea Pig
      Immunogen speciesRat
      Immunogena synthetic peptide (EAYEMPSEEGYQDYEPEA) corresponding to amino acids 123-140 of the C-terminus of rat α-synuclein
      IsotypeIgG
      Speciesbovine, human, mouse, rat
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsCan be used to localize α-synuclein in the central nervous system. For frozen sections, tissue should be fixed in 4% paraformaldehyde and 0.4% picric acid in 0.16 M sodium phosphate buffer, pH 6.9. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesTakeda, A., et al. 1998. Am. J. Pathol. 152, 367.
      Nussbaum, R.L. and Polymeropoulos, M.H., 1997. Hum. Mol. Genet. 6, 1687.
      Wakabayashi, K., et al. 1997. Neurosci. Lett. 239, 45.
      Campion, D., et al. 1995. Genomics 26, 254.
      Iwai, A., et al. 1995. Neuron 14, 467.
      Brookes, A.J. and St. Clair, D. 1994. Trends Neurosci. 17, 404.