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MABS1241 Anti-Synembryn-A/Ric8A Antibody, clone 3E1

Detect Synembryn-A/Ric-8A using this mouse monoclonal Anti-Synembryn-A/Ric8A antibody, clone 3E1, Cat. No. MABS1241, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Synembryn-A/Ric8A Antibody, clone 3E1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Ric8a UniProt Number: Q80ZG1
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MABS1241
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1241
      DescriptionAnti-Synembryn-A/Ric8A Antibody, clone 3E1
      Alternate Names
      • Protein Ric-8A
      • Resistance to inhibitors of cholinesterase 8
      Background InformationSynembryn-A (UniProt: Q80ZG1; also known as Resistance to inhibitors of cholinesterase 8, Protein Ric-8A) is encoded by the Ric8a gene (Gene ID: 293614) in rat species. Resistance to inhibitors of cholinesterase 8 (RIC-8) is conserved in vertebrates and is concentrated in the cytoplasm of neurons. Mammalian Ric-8 proteins are G alpha subunit guanine nucleotide exchange factors. Ric-8A and Ric-8B collectively stimulate nucleotide exchange of all G alpha subunit classes by stabilizing the G alpha nucleotide-free transition state. Ric-8 is reported to directly participate in the post-translational folding of nascent G alpha subunits to promote G-protein biosynthesis. It may facilitate the transition of G alpha from a pre-folded globular state to its native state by promoting the first G alpha guanine nucleotide-binding event. Ric-8 interacts with GDP-bound G alpha proteins, but does not interact with G-alpha proteins when they are in complex with beta and gamma subunits. Overexpression of Ric-8 proteins in HEK293, NIH 3T3, or Sf9 cells is shown to enhance cellular G alpha levels. Embryonic stem cells lacking Ric-8A or Ric-8B produce defective G alpha subunits that turn over at about 10-fold higher rates than in wild-type cells. Ref.: Thomas, C.J. et al. (2011). PLoS ONE 6(8): e23197.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Synembryn-A/Ric-8A using this mouse monoclonal Anti-Synembryn-A/Ric8A antibody, clone 3E1, Cat. No. MABS1241, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Synembryn-A/Ric-8A in human cerebral cortex, human cerebellum, and human testis tissues.

      Western Blotting Analysis: A representative lot detected recombinant Synembryn-A/Ric-8A protein (Chan, P.Y., et al. (2013). Proc. Natl. Acad. Sci. USA. 110(10):3794-3799).
      Biological Information
      ImmunogenA full-length recombinant Ric-8A protein from rat.
      Clone3E1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3E1 detects Ric-8A in human brain.
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ric8a
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~60 kDa observed; 59.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synembryn-A/Ric-8A in 10 µg of human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1241 04054839139154