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NB07 Anti-Synaptobrevin (Ab-2) Mouse mAb (SP11)

Anti-Synaptobrevin (Ab-2) Mouse mAb (SP11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NB07
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes synaptobrevin protein in retinoic acid induced NT2N cells.
      Catalogue NumberNB07
      Brand Family Calbiochem®
      References
      ReferencesHanson, P.I., et al. 1997. Curr. Opin. Neurobiol. 7, 310.
      De Camilli, P. and Takei, K. 1996. Neuron 16, 481.
      Edelmann, L., et al. 1995. EMBO J. 14, 224.
      Sudhof, T.C. 1995. Nature 375, 645.
      Niemann, H., et al. 1994. Trends Cell Biol. 4, 179.
      Greengard, P., et al. 1993. Science 259, 780.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlRetinoic acid induced NT2N cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1:1000)
      Paraffin Sections (see comments, no pre-treatment required)
      Application CommentsThis antibody has also been reported to work for ELISA and paraffin sections. Should this product contain a precipitate, we recommend microcentrifugation before use. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogencrude, human synaptic immunoprecipitate
      ImmunogenHuman
      CloneSP11
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NB07 0

      Documentation

      Anti-Synaptobrevin (Ab-2) Mouse mAb (SP11) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Hanson, P.I., et al. 1997. Curr. Opin. Neurobiol. 7, 310.
      De Camilli, P. and Takei, K. 1996. Neuron 16, 481.
      Edelmann, L., et al. 1995. EMBO J. 14, 224.
      Sudhof, T.C. 1995. Nature 375, 645.
      Niemann, H., et al. 1994. Trends Cell Biol. 4, 179.
      Greengard, P., et al. 1993. Science 259, 780.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      ApplicationELISA (see comments)
      Immunoblotting (1:1000)
      Paraffin Sections (see comments, no pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NSO mouse myeloma cells. Recognizes synaptobrevin.
      BackgroundThe small size (50 nm diameter) and relatively simple protein composition of synaptic vesicles has allowed a detailed characterization of the vesicle components. Synaptic vesicle proteins can be divided into one of two classes; transport proteins involved in neurotransmitter uptake, and trafficking proteins which perform functions in vesicle docking, fusion, and budding. Vesicle proteins are often present in multiple isoforms that are differentially expressed within the nervous system. The function of synaptic vesicle proteins has been derived largely from studies with toxins or by mutational analysis. For example, synaptophysin may function in the process of neurotransmitter release, while synaptobrevin (VAMP) is believed to play a role in synaptic vesicle docking and exocytotic membrane fusion. Synaptobrevin has also been shown to bind synaptophysin with high specificity. Synapsins are highly abundant vesicle membrane proteins that are believed to regulate neurotransmitter release through phosphorylation-dependent interactions with cytoskeletal elements. Other proteins involved in vesicle exocytosis include syntaxin 1 and SNAP-25. In conjunction with synaptobrevin, these proteins form the SNARE (SNAP receptor) complex, which recruits other cytosolic factors including SNAP proteins (soluble NSF attachment proteins) and NSF (N-ethylmaleimide-sensitive factor). The SNARE complex plays an essential role in membrane trafficking.
      HostMouse
      Immunogen speciesHuman
      Immunogencrude, human synaptic immunoprecipitate
      CloneSP11
      IsotypeIgG₁
      Specieshuman, porcine, not rat
      Positive controlRetinoic acid induced NT2N cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody has also been reported to work for ELISA and paraffin sections. Should this product contain a precipitate, we recommend microcentrifugation before use. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHanson, P.I., et al. 1997. Curr. Opin. Neurobiol. 7, 310.
      De Camilli, P. and Takei, K. 1996. Neuron 16, 481.
      Edelmann, L., et al. 1995. EMBO J. 14, 224.
      Sudhof, T.C. 1995. Nature 375, 645.
      Niemann, H., et al. 1994. Trends Cell Biol. 4, 179.
      Greengard, P., et al. 1993. Science 259, 780.