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AB5803 Anti-Sox4 Antibody

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AB5803
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5803
      ReplacesAB9092
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Sox4 Antibody
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sox4 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Sox4 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern blot: 1:200-1:1,000 using ECL.

      Immunocytochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide, amino acids 34-50 of Sox4.
      HostRabbit
      SpecificityRecognizes Sox4. The molecular weight of the protein is 47,258 Daltons.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.
      Gene Symbol
      • SOX4
      • OTTHUMP00000039358
      • EVI16
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q06945 # Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
      SIZE: 474 amino acids; 47263 Da
      SUBUNIT: Interacts with UBE2I.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Testis, brain, and heart.
      SIMILARITY: SwissProt: Q06945 ## Contains 1 HMG box DNA-binding domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

      PREPARATION AND USE:

      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (~10,000G) for 15 minutes to pellet the precipitated antibody product. There will be a whitish pellet near the bottom. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5803 04053252367878

      Documentation

      Anti-Sox4 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Sox4 Antibody Certificates of Analysis

      TitleLot Number
      AB5803RABBIT ANTI-Sox4 - AB5803 AB5803
      RABBIT ANTI-Sox4 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2398817 2398817
      RABBIT ANTI-Sox4 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2453181 2453181
      RABBIT ANTI-Sox4 - 3329064 3329064
      RABBIT ANTI-Sox4 - 3352443 3352443
      RABBIT ANTI-Sox4 - 3507876 3507876
      RABBIT ANTI-Sox4 - 3540426 3540426
      RABBIT ANTI-Sox4 - 3738478 3738478
      RABBIT ANTI-Sox4 - 4123901 4123901
      RABBIT ANTI-Sox4 -2556281 2556281

      References

      Reference overviewPub Med ID
      Cis-regulatory control of corticospinal system development and evolution.
      Shim, S; Kwan, KY; Li, M; Lefebvre, V; Sestan, N
      Nature  486  74-9  2012

      Show Abstract
      22678282 22678282
      Attention deficit/hyperactivity disorder-derived coding variation in the dopamine transporter disrupts microdomain targeting and trafficking regulation.
      Sakrikar, D; Mazei-Robison, MS; Mergy, MA; Richtand, NW; Han, Q; Hamilton, PJ; Bowton, E; Galli, A; Veenstra-Vanderweele, J; Gill, M; Blakely, RD
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  5385-97  2012

      Show Abstract
      22514303 22514303
      The transcription factor Sox4 is a downstream target of signaling by the cytokine TGF-β and suppresses T(H)2 differentiation.
      Kuwahara, M; Yamashita, M; Shinoda, K; Tofukuji, S; Onodera, A; Shinnakasu, R; Motohashi, S; Hosokawa, H; Tumes, D; Iwamura, C; Lefebvre, V; Nakayama, T
      Nature immunology  13  778-86  2012

      Show Abstract
      22751141 22751141
      Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer.
      Andersen, CL; Christensen, LL; Thorsen, K; Schepeler, T; Sørensen, FB; Verspaget, HW; Simon, R; Kruhøffer, M; Aaltonen, LA; Laurberg, S; Ørntoft, TF
      British journal of cancer  100  511-23  2009

      Show Abstract Full Text Article
      19156145 19156145
      Osteopenia, decreased bone formation and impaired osteoblast development in Sox4 heterozygous mice.
      Nissen-Meyer, LS; Jemtland, R; Gautvik, VT; Pedersen, ME; Paro, R; Fortunati, D; Pierroz, DD; Stadelmann, VA; Reppe, S; Reinholt, FP; Del Fattore, A; Rucci, N; Teti, A; Ferrari, S; Gautvik, KM
      Journal of cell science  120  2785-95  2007

      Show Abstract
      17652162 17652162
      Knockdown of Sox4 expression by RNAi induces apoptosis in ACC3 cells.
      Pramoonjago, P; Baras, AS; Moskaluk, CA
      Oncogene  25  5626-39  2006

      Show Abstract
      16636670 16636670

      Data Sheet

      Title
      RABBIT ANTI-Sox4 AFFINITY PURIFIED POLYCLONAL ANTIBODY