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MABE126 Anti-SR protein family Antibody, clone 16H3

Anti-SR protein family Antibody, clone 16H3 is a Mouse Monoclonal Antibody for detection of SR protein family also known as arginine/serine-rich splicing factor 6 & has been validated in WB, IP, ICC.

Anti-SR protein family Antibody, clone 16H3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: SRP55, B52, SFRS6 UniProt Number: Q13247
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MABE126
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE126
      DescriptionAnti-SR protein family Antibody, clone 16H3
      Alternate Names
      • splicing factor, arginine/serine-rich 6
      • arginine/serine-rich splicing factor 6
      • splicing factor, arginine/serine-rich, 55 kDa
      • Pre-mRNA-splicing factor SRP55
      • pre-mRNA splicing factor SRP55
      Background InformationSer-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including the identification and appropriation of splice-sites and the manipulation of alternative splicing regulation.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SR protein family Antibody, clone 16H3 is a Mouse Monoclonal Antibody for detection of SR protein family also known as arginine/serine-rich splicing factor 6 & has been validated in WB, IP, ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Neugebauer, KM, et al. (1995).

      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Neugebauer, KM, et al. (1995).
      Biological Information
      ImmunogenDephosphorylated bovine SRp55 and GST-tagged recombinant dephosphorylated SRp55 (Neugebauer, KM et. al 1995).
      Clone16H3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the four nuclear proteins SRp75, SRp55, SRp40, and SRp20, plus ~20 other prominent nuclear proteins believed to correspond to the SR family.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human.
      Predicted to react with mouse and rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants.
      Gene Symbol
      • SRP55
      • B52
      • SFRS6
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in constitutive splicing and can modulate the selection of alternative splice sites.

      SUBUNIT STRUCTURE: Binds SFRS12.

      SUBCELLULAR LOCATION: Nucleus.

      PTM: Extensively phosphorylated on serine residues in the RS domain (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.

      Contains 2 RRM (RNA recognition motif) domains.
      Molecular WeightMultiple SR protein family members detected. Reported to predominantly detect SRp75, SRp55, SRp40, SRp30, SRp20 (Neugebauer, KM, et al. (1995).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected SR proteins in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE126 04053252553363

      Documentation

      Anti-SR protein family Antibody, clone 16H3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SR protein family Antibody, clone 16H3 Certificates of Analysis

      TitleLot Number
      Anti-SR protein family, clone 16H3 2479821
      Anti-SR protein family, clone 16H3 - 2119212 2119212
      Anti-SR protein family, clone 16H3 - 2301297 2301297
      Anti-SR protein family, clone 16H3 - 3484053 3484053
      Anti-SR protein family, clone 16H3 - 3537153 3537153
      Anti-SR protein family, clone 16H3 - 3587250 3587250
      Anti-SR protein family, clone 16H3 - 3743410 3743410
      Anti-SR protein family, clone 16H3 - 3835494 3835494
      Anti-SR protein family, clone 16H3 - 4070119 4070119
      Anti-SR protein family, clone 16H3 - 4195018 4195018
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies