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DR1056 Anti-SQSTM1 Mouse pAb

This Anti-SQSTM1 Mouse pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of SQSTM1.

Anti-SQSTM1 Mouse pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Sequestosome 1

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DR1056
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMPolyclonal Antibody
      Description
      OverviewRecognizes the ~60 kDa SQSTM1 protein in HeLa cells.
      Catalogue NumberDR1056
      Brand Family Calbiochem®
      SynonymsAnti-Sequestosome 1
      Application Data
      Detection of human SQSTM1 by immunoblotting. Samples: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human SQSTM1 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-SQSTM1 Mouse pAb (Cat. No. DR1056) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SQSTM1 by immunocytochemistry. Samples: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse pAb (Cat. No. DR1056) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length human SQSTM1 (aa 1-440)
      ImmunogenHuman
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1056 0

      Documentation

      Anti-SQSTM1 Mouse pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Sequestosome 1
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human SQSTM1 by immunoblotting. Samples: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human SQSTM1 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-SQSTM1 Mouse pAb (Cat. No. DR1056) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SQSTM1 by immunocytochemistry. Samples: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse pAb (Cat. No. DR1056) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse polyclonal antibody. Recognizes the ~60 kDa SQSTM1 protein.
      BackgroundSQSTM1 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals.
      HostMouse
      Immunogen speciesHuman
      ImmunogenFull-length human SQSTM1 (aa 1-440)
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa cells
      Negative control293T cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review