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DR1035 Anti-SMG1 Rabbit pAb

This Anti-SMG1 Rabbit pAb is validated for use in Immunoblotting for the detection of SMG1.

Anti-SMG1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ATM-Related Kinase, Anti-LIP, Anti-61E3.4, Anti-KIAA0421, Anti-ATX, Anti-PI-3-Kinase-Related Kinase SMG1

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DR1035
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~340 kDa SMG protein in HeLa cell nuclear extracts.
      Catalogue NumberDR1035
      Brand Family Calbiochem®
      SynonymsAnti-ATM-Related Kinase, Anti-LIP, Anti-61E3.4, Anti-KIAA0421, Anti-ATX, Anti-PI-3-Kinase-Related Kinase SMG1
      Application Data
      Detection of human SMG-1 by immunoblotting. Sample: Whole cell lysate (50 μg) from HeLa cells. Primary antibody: Anti-SMG-1 Rabbit pAb (Cat. No. DR1035) (0.1 μg/ml). Detection: chemiluminescence.
      References
      ReferencesAbraham, R.T., 2004. DNA Repair (Amst.) 3, 919.
      Brumbaugh, K.M., et al. 2004. Mol. Cell. 14, 585.
      Yamashita, A., et al. 2001. Genes Dev. 15, 2215.
      Product Information
      FormLiquid
      FormulationIn 100 mM NaCl, 10 mM phosphate, pH 7.2.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (0.1 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 1625-167 of human SMG1
      ImmunogenHuman
      Epitopewithin amino acids 1625-1675
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1035 0

      Documentation

      Anti-SMG1 Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SMG1 Rabbit pAb Certificates of Analysis

      TitleLot Number
      DR1035

      References

      Reference overview
      Abraham, R.T., 2004. DNA Repair (Amst.) 3, 919.
      Brumbaugh, K.M., et al. 2004. Mol. Cell. 14, 585.
      Yamashita, A., et al. 2001. Genes Dev. 15, 2215.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-June-2013 JSW
      SynonymsAnti-ATM-Related Kinase, Anti-LIP, Anti-61E3.4, Anti-KIAA0421, Anti-ATX, Anti-PI-3-Kinase-Related Kinase SMG1
      ApplicationImmunoblotting (0.1 µg/ml)
      Application Data
      Detection of human SMG-1 by immunoblotting. Sample: Whole cell lysate (50 μg) from HeLa cells. Primary antibody: Anti-SMG-1 Rabbit pAb (Cat. No. DR1035) (0.1 μg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~340 kDa SMG protein.
      BackgroundSMG-1 (ACC# NP 055907), a PIKK family member, was named based on its homology to the Caenorhabditis elegans protein CeSMG-1. SMG-1 functions in a RNA surveillance pathway termed nonsense-mediated mRNA decay (NMD). NMD mediates the rapid degradation of mRNAs bearing premature termination codons (PTCs), which threaten cell survival by producing truncated, potentially dominant-interfering proteins. Inhibition of SMG-1 activity in mammalian cells leads to suppression of NMD. SMG-1 regulates NMD in part through the phosphorylation of hUpf1, a conserved component of the NMD machinery in metazoan cells. SMG-1 phosphorylates at least four sites in the carboxy-terminal, S/T-Q-rich domain of hUpf1, and mutational analysis indicated that these phosphorylation events are important for NMD activity. SMG-1 has also been called ATX to acknowledge its similarity to the genome surveillance kinases ATM and ATR. In addition to its critical role in NMD, SMG-1 is centrally involved in the regulation of the genotoxic stress response machinery in mammalian cells. Cellular exposure to ultraviolet (UV) light or IR stimulates SMG-1 kinase activity and SMG-1 function is required for maximal p53 phosphorylation and stabilization in radiation-damaged cells. Moreover, SMG-1-deficient cells are sensitized to IR-induced stress.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 1625-167 of human SMG1
      Epitopewithin amino acids 1625-1675
      IsotypeIgG
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn 100 mM NaCl, 10 mM phosphate, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesAbraham, R.T., 2004. DNA Repair (Amst.) 3, 919.
      Brumbaugh, K.M., et al. 2004. Mol. Cell. 14, 585.
      Yamashita, A., et al. 2001. Genes Dev. 15, 2215.