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OP66 Anti-Rb (Ab-5) Mouse mAb (LM95.1)

This Anti-Rb (Ab-5) Mouse mAb (LM95.1) is validated for use in Immunoblotting, Paraffin Sections for the detection of Rb (Ab-5).

Anti-Rb (Ab-5) Mouse mAb (LM95.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Retinoblastoma Associated Protein, Anti-pp105

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OP66
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~105 kDa Rb protein. Sold under license of U.S. Patent 5,853,988 and corresponding patents.
      Catalogue NumberOP66
      Brand Family Calbiochem®
      SynonymsAnti-Retinoblastoma Associated Protein, Anti-pp105
      References
      ReferencesDyson, N., et al. 1992. J. Virol. 66, 4606.
      Dyson, N., et al. 1990. J. Virol. 64, 1353.
      Horowitz, J.M., et al. 1989. Science 243, 937.
      Whyte, P., et al. 1988. Nature 334, 124.
      Klein, G. 1987. Science 238, 1539.
      Lee, W.H., et al. 1987. Science 235, 1394.
      Lee, W.H., et al. 1987. Nature 329, 642.
      Knudson, A.G. 1971. Proc. Natl. Acad. Sci. USA 68, 820.
      Product Information
      DeclarationSold under license of U.S. Patent 5,853,988 and corresponding patents.
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlSaos cells
      Positive controlML-1 cells or normal colon tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Thomas, D.M., et al. 2001. Mol. Cell 8, 303. Paraffin Sections Ioachim, E., et al. 1999. Virchows Arch. 434, 45.
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml; see application references)
      Paraffin Sections (3 µg/ml, see comments and application references)
      Application CommentsPre-treatment with heat or pressure cooker may improve staining of some tissue specimens; this should be determined for each specimen. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human Rb
      ImmunogenHuman
      Epitopewithin a 56 kDa C-terminal fragment
      CloneLM95.1
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP66 0

      Documentation

      Anti-Rb (Ab-5) Mouse mAb (LM95.1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Rb (Ab-5) Mouse mAb (LM95.1) Certificates of Analysis

      TitleLot Number
      OP66

      References

      Reference overview
      Dyson, N., et al. 1992. J. Virol. 66, 4606.
      Dyson, N., et al. 1990. J. Virol. 64, 1353.
      Horowitz, J.M., et al. 1989. Science 243, 937.
      Whyte, P., et al. 1988. Nature 334, 124.
      Klein, G. 1987. Science 238, 1539.
      Lee, W.H., et al. 1987. Science 235, 1394.
      Lee, W.H., et al. 1987. Nature 329, 642.
      Knudson, A.G. 1971. Proc. Natl. Acad. Sci. USA 68, 820.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-November-2008 JSW
      SynonymsAnti-Retinoblastoma Associated Protein, Anti-pp105
      ApplicationImmunoblotting (1 µg/ml; see application references)
      Paraffin Sections (3 µg/ml, see comments and application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~105 kDa Rb protein.
      BackgroundCertain genes have been identified whose function appears to be the supression of tumor formation and are therefore classified as tumor suppressor genes. One such gene has been linked to the hereditary cancer retinoblastoma. The retino-blastoma (Rb) gene encodes a protein of 928 amino acids which is found predominantly in the nucleus and has the capability of binding to DNA. The Rb protein may be modified by phosphorylation but while there are several potential sites for N-linked glycosylation, no modification has been detected. The Rb protein has been reported as having apparent molecular weights of ~105 kDa, ~100-114 kDa, and ~102 or ~104 kDa which are antigenically related. The Rb protein has been detect in at least 20 different common laboratory cell lines with the exception of retinoblastoma cells. The malignant trans-formation of cells is caused by inactivation of the gene by mutation but also by inactivation of the Rb protein resulting from binding to the adenovirus E1A protein or SV40 T antigen.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human Rb
      Epitopewithin a 56 kDa C-terminal fragment
      CloneLM95.1
      IsotypeIgG₁
      Specieshuman
      Positive controlML-1 cells or normal colon tissue
      Negative controlSaos cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsPre-treatment with heat or pressure cooker may improve staining of some tissue specimens; this should be determined for each specimen. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDyson, N., et al. 1992. J. Virol. 66, 4606.
      Dyson, N., et al. 1990. J. Virol. 64, 1353.
      Horowitz, J.M., et al. 1989. Science 243, 937.
      Whyte, P., et al. 1988. Nature 334, 124.
      Klein, G. 1987. Science 238, 1539.
      Lee, W.H., et al. 1987. Science 235, 1394.
      Lee, W.H., et al. 1987. Nature 329, 642.
      Knudson, A.G. 1971. Proc. Natl. Acad. Sci. USA 68, 820.
      Application referencesImmunoblotting Thomas, D.M., et al. 2001. Mol. Cell 8, 303. Paraffin Sections Ioachim, E., et al. 1999. Virchows Arch. 434, 45.