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MABN2286-25UL Anti-Rai Antibody, clone IF15

Anti-Rai, clone IF15, Cat. No. MABN2286, is a mouse monoclonal antibody that detects SHC-transforming protein 3 (Rai) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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MABN2286-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2286-25UL
      DescriptionAnti-Rai Antibody, clone IF15
      Alternate Names
      • SHC-transforming protein 3
      • Neuronal Shc
      • N-Shc
      • Protein Rai
      • SHC-transforming protein C
      • Src homology 2 domain-containing-transforming protein C3
      • SH2 domain protein C3
      Background InformationSHC-transforming protein 3 (UniProt: Q92529; also known as Neuronal Shc, N-Shc, Protein Rai, SHC-transforming protein C, Src homology 2 domain-containing-transforming protein C3, SH2 domain protein C3, Rai) is encoded by the SHC3 (also known as NSHC, SHCC) gene (Gene ID:53358) in human. Rai protein is mainly expressed in the brain and its higher expression is observed in cerebral cortex, frontal and temporal lobes, hippocampus, and occipital pole. Its expression is also reported in the enteric glial cells in the human gut. It serves as a signaling adapter and is involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons. Rai also serves as a physiological substrate of the receptor tyrosine kinase Ret and increases Ret-dependent survival via PI3 kinase/Akt pathway activation. It also potentiates MAP kinase signaling. Rai activation also plays a role in long-term potential and is neuroprotective against certain ischemia and oxidative stress. Evolutionarily it correlates well with vertebrate brain development. Its reduced levels are observed in brain of Alzheimer s disease subjects. Two isoforms of Rai have been described (p64 and p52) that are produced by alternative splicing. (Ref.: Triaca, V., et al. (2018). Aging. 10(1); 5-6; Sagi, O., et al. (2015). Ageing Res. Rev. 19; 34-42; Villanacci, V., et al. (2008). Neurogastroenterol. Motil. 20(3); 206-212).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Rai, clone IF15, Cat. No. MABN2286, is a mouse monoclonal antibody that detects SHC-transforming protein 3 (Rai) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected Rai in Immunohistochemistry applications (Villanacci, V., et. al. (2008). Neurogastroenterol Motil. 20(3):206-12).

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rai in U2OS cells.

      Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Rai in human esophagus, stomach, and colon tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant fragment corresponding to 164 amino acids CH1 domain of human SHC-transforming protein 3 (Rai).
      EpitopeC-terminal half
      CloneIF15-E2
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone IF15-E2 is a mouse monoclonal antibody that detects SHC-transforming protein 3/RAI. It targets an epitope within the CH1 domain from the C-terminal half.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SHC3
      • NSHC
      • SHCC
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~58 kDa observed; 64.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human brain tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Rai in Human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2286-25UL 04065266553901