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MABS1182 Anti-RagA/B Antibody, clone 1H7.1

Detect RagA and RagB using this mouse monoclonal Anti-RagA/B, clone 1H7.1, Cat. No. MABS1182, validated for use in ELISA, Immunocytochemistry, and Western Blotting.

Anti-RagA/B Antibody, clone 1H7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: RRAGA UniProt Number: Q7L523
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MABS1182
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, ICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1182
      DescriptionAnti-RagA/B Antibody, clone 1H7.1
      Alternate Names
      • Ras-related GTP-binding protein A
      • Adenovirus E3 14.7 kDa-interacting protein 1
      • FIP-1
      • Rag A
      • RagA
      Background InformationRas-related GTP-binding protein A (UniProt Q7L523; also known as Adenovirus E3 14.7 kDa-interacting protein 1, FIP-1. Rag A, RagA) is encoded by the RRAGA gene (Gene ID 10670) in human. Mammalian cells express four Rag GTPases that mediate amino acid sufficiency-dependent mTORC1 activation to support cell growth and proliferation. Rag GTPases assemble into heterodimers containing GTP-loaded RagA or RagB partnered with GDP-loaded RagC or RagD. Promoted by signal from an upstream amino acid-responsive supercomplex, the active Rag heterodimer accumulates on the cytoplasmic surface of the lysosome, where it recruits mTORC1 and initiates its activation. The pentameric Ragulator complex tethers Rag heterodimers to the lysosomal membrane and acts as RagA/B guanine nucleotide exchange factor (GEF), while the heterotrimeric GATOR1 complex and the heterodimeric FNIP-Folliculin complex function as RagA/B and RagC/D GTPase activating protein (GAP), respectively. Both lysosomal amino acid sensors, such as the multisubunit vacuolar ATPase (v-ATPase) and lysosomal amino acid transporter(s), and cytoplasmic amino acid sensors, such as the leucyl-tRNA synthetase (LeuRS), are part of the upstream amino acid-responsive supercomplex.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect RagA and RagB using this mouse monoclonal Anti-RagA/B, clone 1H7.1, Cat. No. MABS1182, validated for use in ELISA, Immunocytochemistry, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected exogenously overexpressed human RagA in 1 µg of lysate from transfected HEK293T cells.

      Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected RagA/B immunoreactivity in HUVECs.

      ELISA Analysis: Clone 1H7.1 hybridoma culture supernatant detected exogenously overexpressed human RagA and RagB in lysates (100 ng/well) from transfected HEK293T cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal end sequence of human/mouse/rat RagA.
      Clone1H7.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1H7.1 targets an epitope within the C-terminal end sequence highly conserved between RagA and RagB.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Rat, Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RRAGA
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~36 kDa observed. 36.57 kDa (human/mouse/rat/bovine RagA), 43.25/40.17 kDa (human RagB Long/Short isoform), 43.19 kDa (mouse RagB), 43.19/40.11 (rat RagB Long/Short isoform) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected RagA in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1182 04054839091360